anti-Dog (Canine) CD11b antibody for Cytometry by Time of Flight

Recommended CD11b Antibody

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Epitope
AA 17-1105
23
16
13
11
10
8
8
6
6
5
5
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
656
432
146
80
35
33
30
25
18
13
10
10
8
8
6
5
4
4
2
Host
Goat
475
346
175
16
6
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
139
116
90
62
17
16
14
12
11
11
10
10
10
9
9
6
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
782
247
208
155
139
111
75
60
49
32
20
14
13
11
5
5
4
4
3
2
2
1
1
1
1
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Catalog No. ABIN4899289
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General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope AA 17-1105
(23), (16), (13), (11), (10), (8), (8), (6), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human
(656), (432), (146), (80), (35), (33), (30), (25), (18), (13), (10), (10), (8), (8), (6), (5), (4), (4), (2)
Host Goat
(475), (346), (175), (16), (6), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(139), (116), (90), (62), (17), (16), (14), (12), (11), (11), (10), (10), (10), (9), (9), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
(782), (247), (208), (155), (139), (111), (75), (60), (49), (32), (20), (14), (13), (11), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN4899289) Images
Specificity Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In direct ELISAs, less than 5 % cross-reactivity with recombinant mouse Integrin alpha M and recombinant human Integrin alpha E is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M, Phe17-Asn1105, Accession # NP_001139280
Isotype IgG
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN4899289) Images back to top
Antigen
Alternative Name CD11b/Integrin alpha M (ITGAM Antibody Abstract)
Background Alternative Names: antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, CD11Bintegrin, alpha M (complement component receptor 3, alpha, also known as CD11b(p170), macrophage antigen alpha polypeptide), Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3AMGC117044, Integrin alpha M, integrin alpha-M, integrin, alpha M (complement component 3 receptor 3 subunit), ITGAM, Leukocyte adhesion receptor MO1, MAC-1, MAC1A, macrophage antigen alpha polypeptide, MO1A, Neutrophil adherence receptor, neutrophil adherence receptor alpha-M subunit, SLEB6
Gene ID 3684
NCBI Accession NP_001139280
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN4899289) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN4899289) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD11b antibody (ABIN4899289)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Jerke, Rolle, Dittmar, Bayat, Santoso, Sporbert, Luft, Kettritz: "Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation." in: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7070-81, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN4899289) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) (AA 17-1105) antibody (ABIN4899289) Detection of CD11b/Integrin alpha M in Canine PBMCs by Flow Cytometry. Canine periphe...