anti-Human CD11b antibody for Immunohistochemistry (Frozen Sections)

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Human, Mouse (Murine)
760
499
173
104
37
33
32
25
14
13
10
10
9
8
6
5
4
4
2
2
Host
Rat
539
407
163
16
5
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
147
127
98
65
18
18
16
16
14
14
13
13
12
11
11
9
8
7
7
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
911
270
219
203
169
160
92
91
88
62
26
20
13
13
9
8
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2688906
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN1177001 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8
8.5 ABIN118516 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 5
8.5 ABIN118517 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
8.5 ABIN118513 FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
7 ABIN573960 FACS IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 0
7 ABIN379956 IHC IHC (fro) Mouse Log in to see X-5 0
5.5 ABIN319676 FACS IHC (fro) Mouse IgG1 Log in to see ICRF44 4
5.5 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
5.5 ABIN2478219 IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
5.5 ABIN2478233 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 4
5.5 ABIN2478215 IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 4
5.5 ABIN2478217 IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 4
5.5 ABIN2688915 FACS IHC (fro) WB Mouse IgG1, kappa Log in to see ICRF44 1
4 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
4 ABIN120130 FACS IHC (fro) IF IP Rat IgG2b Log in to see 5C6 13
4 ABIN1724066 IHC (fro) FACS IHC (p) IP Rat IgG2b Log in to see M1-70-15 0
4 ABIN205315 FACS IHC IHC (fro) Mouse IgG1 Log in to see ICRF44 0
1 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
1 ABIN320196 FACS IHC (fro) IP Rat IgG2b Log in to see 5C6 13
1 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine)
(760), (499), (173), (104), (37), (33), (32), (25), (14), (13), (10), (10), (9), (8), (6), (5), (4), (4), (2), (2)
Host Rat
(539), (407), (163), (16), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(147), (127), (98), (65), (18), (18), (16), (16), (14), (14), (13), (13), (12), (11), (11), (9), (8), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(911), (270), (219), (203), (169), (160), (92), (91), (88), (62), (26), (20), (13), (13), (9), (8), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN2688906) Images
Brand BD Pharmingen™
Characteristics The M1/70 antibody reacts with the 170 kDa α[M] chain of Mac-1 (CD11b/CD18, α[M]β2 integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11b on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified Rat IgG2b, κ isotype control A95-1 (Cat. No. 553986, left panel) or purified M1/70 monoclonal antibodies (right panel), followed by FITC-conjugated goat anti-rat Ig (Cat. No. 554016, both panels). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD11b - Purified - Clone M1/70 - Isotype Rat IgG2b, κ - Reactivity Ms, Hu - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen C57BL/10 splenic T cells and concanavalin A-activated C57BL/10 splenocytes
Clone M1-70
Isotype IgG2b, kappa
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN2688906) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Synonyms: Integrin α[M] chain, Mac-1 α chain, CR3
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN2688906) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN2688906) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD11b antibody (ABIN2688906)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, 1996

Lub, van Kooyk, Figdor: "Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54)." in: Journal of leukocyte biology, Vol. 59, Issue 5, pp. 648-55, 1996

Kishimoto, Jutila, Berg, Butcher: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." in: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, 1989

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983

Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983

Springer, Galfré, Secher, Milstein: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978