anti-Human CD11b antibody for Immunoprecipitation

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Human
752
493
173
103
37
33
32
25
14
13
10
10
9
9
6
5
4
4
2
2
Host
Mouse
532
403
164
17
5
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to APC
144
125
97
62
17
16
15
15
14
13
12
12
12
11
11
9
7
7
7
6
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
905
264
215
201
172
163
90
89
88
61
21
20
13
13
8
8
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1302538
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.967865 ABIN93959 FACS IP FITC Mouse IgG2a Log in to see MEM-174 5
29.967865 ABIN93958 FACS IP Biotin Mouse IgG2a Log in to see MEM-174 5
29.967865 ABIN93960 FACS IP PE Mouse IgG2a Log in to see MEM-174 5
29.967865 ABIN93956 FACS IP Mouse IgG2a Log in to see MEM-174 5
29.967865 ABIN125683 FACS IP Mouse IgG2a Log in to see MEM-174 5
29.575207 ABIN1690733 FACS Func IHC (f) IP APC Rat IgG2b Log in to see M1-70 10
22.075207 ABIN457369 FACS Func IHC (f) IP PE Rat IgG2b Log in to see M1-70 9
22.075207 ABIN457346 FACS Func IHC (f) IP FITC Rat IgG2b Log in to see M1-70 8
20.575207 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 Log in to see ICRF44 4
20.575207 ABIN2749103 Func IHC (f) ICC FACS IP PE Mouse IgG1 Log in to see ICRF44 4
17.575207 ABIN457402 FACS Func IHC (f) IP Rat IgG2b Log in to see M1-70 5
17.575207 ABIN1981858 Func IHC (f) ICC FACS IP Biotin Mouse IgG1 Log in to see ICRF44 4
17.575207 ABIN1027692 FACS Func ICC IHC (f) IP Mouse IgG1 Log in to see ICRF44 3
17.575207 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 Log in to see ICRF44 3
13.39266 ABIN532755 FACS IP Mouse IgG2a Log in to see MEM-174 0
13.075206 ABIN4888496 FACS Func IP PE Mouse IgG1 Activation Site Log in to see CBRM1-5 0
13.075206 ABIN5633268 FACS Func IHC (f) IP Rat IgG2b Log in to see M1-70 0
13.075206 ABIN4888495 FACS Func IP Mouse IgG1 Activation Site Log in to see CBRM1-5 0
13.075206 ABIN2135907 FACS IP Mouse IgG1 Log in to see Bear-1 0
10 ABIN2688906 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human
(752), (493), (173), (103), (37), (33), (32), (25), (14), (13), (10), (10), (9), (9), (6), (5), (4), (4), (2), (2)
Host Mouse
(532), (403), (164), (17), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to APC
(144), (125), (97), (62), (17), (16), (15), (15), (14), (13), (12), (12), (12), (11), (11), (9), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP)
(905), (264), (215), (201), (172), (163), (90), (89), (88), (61), (21), (20), (13), (13), (8), (8), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1302538) Images
Specificity The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
HLDA VI, WS Code BP 310
HLDA VI, WS Code M 18
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen Human granulocytes
Clone MEM-174
Isotype IgG2a
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN1302538) Images back to top
Antigen
Alternative Name ITGAM (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Gene ID 3684
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN1302538) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN1302538) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD11b antibody (ABIN1302538)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Background publications

Lawrence, Srikumaran: "CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization." in: Veterinary immunology and immunopathology, Vol. 119, Issue 3-4, pp. 287-98, 2007

Akramiene, Kondrotas, Didziapetriene, Kevelaitis: "Effects of beta-glucans on the immune system." in: Medicina (Kaunas, Lithuania), Vol. 43, Issue 8, pp. 597-606, 2007

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1302538) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-Integrin alpha M (ITGAM) antibody (APC) (ABIN1302538) Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC is...
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (APC) (ABIN1302538) Surface staining of human peripheral blood with anti-human CD11b (MEM-174) APC.