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|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Conjugate||This CD11b antibody is conjugated to APC Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP)
|4 references available|
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Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1302538) Images
The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
HLDA VI, WS Code BP 310
HLDA VI, WS Code M 18
|Characteristics||The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN1302538) Images back to top
|Alternative Name||ITGAM (ITGAM Antibody Abstract)|
|Background||CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.|
|Pathways||Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN1302538) Images back to top
|Application Notes||The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN1302538) Images back to top
|Reconstitution||No reconstitution is necessary.|
|Buffer||The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Do not freeze.
Avoid prolonged exposure to light.
|Storage Comment||Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.|
References for anti-CD11b antibody (ABIN1302538)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
|Product cited in:||
Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015
Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007
Lawrence, Srikumaran: "CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization." in: Veterinary immunology and immunopathology, Vol. 119, Issue 3-4, pp. 287-98, 2007
Akramiene, Kondrotas, Didziapetriene, Kevelaitis: "Effects of beta-glucans on the immune system." in: Medicina (Kaunas, Lithuania), Vol. 43, Issue 8, pp. 597-606, 2007
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1302538) back to top