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|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Reactivity||Human, Monkey Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|4 references available|
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Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN118517) Images
|Specificity||This antibody recognises the CD11b cell surface glycoprotein. This antibody blocks some functions of CD11b, although full definition is still to be completed.|
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Rheumatoid synovial cells and human mocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN118517) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
|Background||CD11b is a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor|
|Pathways||Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN118517) Images back to top
Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN118517) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD11b antibody (ABIN118517)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
|Product cited in:||
Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, 2006
Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000
Dransfield, Cabañas, Barrett, Hogg: "Interaction of leukocyte integrins with ligand is necessary but not sufficient for function." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1527-35, 1992
Malhotra, Hogg, Sim: "Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3)." in: European journal of immunology, Vol. 16, Issue 9, pp. 1117-23, 1986
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN118517) back to top