anti-Mouse (Murine) CD11b antibody for Immunohistochemistry (Frozen Sections)

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Epitope
Internal Region, AA 250-350
38
19
17
16
13
13
10
8
8
8
7
6
5
5
5
5
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
759
498
172
104
37
33
32
25
14
13
10
10
9
8
6
5
4
4
2
2
Host
Rabbit
539
408
162
16
4
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
147
127
98
65
18
18
16
16
14
14
13
13
12
11
11
9
8
7
7
6
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
911
268
220
202
168
160
92
90
88
62
26
21
13
13
9
8
5
4
4
3
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN409930
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
1 ABIN120130 FACS IHC (fro) IF IP Rat IgG2b Log in to see 5C6 13
1 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
1 ABIN320196 FACS IHC (fro) IP Rat IgG2b Log in to see 5C6 13
1 ABIN370850 FACS IHC (fro) IP Rat IgG2a Log in to see 3A33 11
1 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12
1 ABIN370845 FACS IHC (fro) IP Biotin Rat IgG2a Log in to see 3A33 11
1 ABIN370849 FACS IHC (fro) IP Rat IgG2a Log in to see 3A33 11
1 ABIN573960 FACS IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 0
1 ABIN2688906 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8
1 ABIN1177001 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8
1 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6
1 ABIN4289459 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 488 Rat IgG2b Log in to see M1-70-15 0
1 ABIN1724066 IHC (fro) FACS IHC (p) IP Rat IgG2b Log in to see M1-70-15 0
1 ABIN2688908 IHC (f) IHC (fro) FACS Rat IgG2b, kappa Log in to see M1-70 4
1 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN2478219 IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN490173 BR FACS Func IHC IHC (fro) IP Neut Rat IgG2b Log in to see M1-70 0
1 ABIN4289434 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 0
1 ABIN4289435 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 0

General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope Internal Region, AA 250-350
(38), (19), (17), (16), (13), (13), (10), (8), (8), (8), (7), (6), (5), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(759), (498), (172), (104), (37), (33), (32), (25), (14), (13), (10), (10), (9), (8), (6), (5), (4), (4), (2), (2)
Host Rabbit
(539), (408), (162), (16), (4), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(147), (127), (98), (65), (18), (18), (16), (16), (14), (14), (13), (13), (12), (11), (11), (9), (8), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(911), (268), (220), (202), (168), (160), (92), (90), (88), (62), (26), (21), (13), (13), (9), (8), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 31 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN409930) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN409930) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Gene Symbol: ITGAM
Molecular Weight Theoretical MW: 127 kDa
Gene ID 3684
UniProt P11215, P05555
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN409930) Images back to top
Application Notes Western Blot 2 μg/mL, Simple Western 1:50, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry-Paraffin 1:400, Immunohistochemistry-FrozenThis CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN409930) Images back to top
Format Liquid
Concentration 1.00 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-CD11b antibody (ABIN409930)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Schmidt, Hobbing, Donninger, Clark: "RASSF1A Deficiency Enhances RAS-Driven Lung Tumorigenesis." in: Cancer research, Vol. 78, Issue 10, pp. 2614-2623, 2018

Periyasamy, Thangaraj, Guo, Hu, Callen, Buch: "Epigenetic Promoter DNA Methylation of miR-124 Promotes HIV-1 Tat-Mediated Microglial Activation via MECP2-STAT3 Axis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 38, Issue 23, pp. 5367-5383, 2018

Xu, Xu, Ge, Tian, Zhao, Guo: "Anti-inflammatory effect of low molecular weight fucoidan from Saccharina japonica on atherosclerosis in apoE-knockout mice." in: International journal of biological macromolecules, Vol. 118, Issue Pt A, pp. 365-374, 2018

Baranova, Souza, Bocharov, Vishnyakova, Hu, Vaisman, Amar, Chen, Remaley, Patterson, Yuen, Star, Eggerman: "Human SR-BII mediates SAA uptake and contributes to SAA pro-inflammatory signaling in vitro and in vivo." in: PLoS ONE, Vol. 12, Issue 4, pp. e0175824, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Li, Lu, Sheng, Zhu, Sun, Zhou, Yang, Xue, Chen, Tian, Du, Yan, Zhu, Xing, Li, Lin, Qiu, Su, Huang, Yan, Yin: "Hexokinase 2-dependent hyperglycolysis driving microglial activation contributes to ischemic brain injury." in: Journal of neurochemistry, Vol. 144, Issue 2, pp. 186-200, 2017 (Sample species: Rat (Rattus)).

Gao, Gülcüler, Golbach, Block, Zarbock, Martin-Villalba: "Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion." in: eLife, Vol. 5, 2017

Kim, Kang, Choi, Joo, Kim, Song: "Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-β2 induces anti-tumor immune activation." in: Oncotarget, Vol. 8, Issue 9, pp. 15858-15877, 2017

Algieri, Rodriguez-Nogales, Garrido-Mesa, Camuesco, Vezza, Garrido-Mesa, Utrilla, Rodriguez-Cabezas, Pischel, Galvez: "Intestinal anti-inflammatory activity of calcium pyruvate in the TNBS model of rat colitis: Comparison with ethyl pyruvate." in: Biochemical pharmacology, Vol. 103, pp. 53-63, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Makar, Nimmagadda, Singh, Lam, Mubariz, Judge, Trisler, Bever: "TrkB agonist, 7,8-dihydroxyflavone, reduces the clinical and pathological severity of a murine model of multiple sclerosis." in: Journal of neuroimmunology, Vol. 292, pp. 9-20, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kim, Kim, Wu, Park, Ha, Kim, Lee, Lee, Kwon: "Extracellular Release of CD11b by TLR9 Stimulation in Macrophages." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150677, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Xu, Meng, Erben, Wang, Glauben, Kühl, Wu, Ma, Hu, Wang, Sun, Jia, Wu, Chen, Siegmund, Qin: "Myeloid-derived suppressor cells promote B-cell production of IgA in a TNFR2-dependent manner." in: Cellular & molecular immunology, 2016

Yang, Scott, Monickaraj, Xu, Ardekani, Nitta, Cabrera, McGuire, Mohideen, Das, Ghosh: "Basement membrane stiffening promotes retinal endothelial activation associated with diabetes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 2, pp. 601-11, 2016

Rawat, Spector: "Development and characterization of a human microglia cell model of HIV-1 infection." in: Journal of neurovirology, Vol. 23, Issue 1, pp. 33-46, 2016

Ulasov, Borovjagin, Kaverina, Schroeder, Shah, Lin, Baryshnikov, Cobbs: "MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo." in: Cancer letters, Vol. 365, Issue 2, pp. 240-50, 2015

Stiess, Wegehingel, Nguyen, Nickel, Bradke, Cambridge: "A Dual SILAC Proteomic Labeling Strategy for Quantifying Constitutive and Cell-Cell Induced Protein Secretion." in: Journal of proteome research, Vol. 14, Issue 8, pp. 3229-38, 2015 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Makar, Gerzanich, Nimmagadda, Jain, Lam, Mubariz, Trisler, Ivanova, Woo, Kwon, Bryan, Bever, Simard: "Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 12, pp. 210, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Sakaguchi, Shono, Suzuki, Sawano, Anderson, Do, Ohtsubo, Mizunoya, Sato, Nakamura, Furuse, Yamada, Ikeuchi, Tatsumi: "Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle." in: The international journal of biochemistry & cell biology, Vol. 54, pp. 272-85, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Sawano, Suzuki, Do, Ohtsubo, Mizunoya, Ikeuchi, Tatsumi: "Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 85, Issue 12, pp. 994-1000, 2014

Gil-Pisa, Cebrián, Ortega, Meana, Sulzer: "Cytokine pathway disruption in a mouse model of schizophrenia induced by Munc18-1a overexpression in the brain." in: Journal of neuroinflammation, Vol. 11, pp. 128, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Shono, Sakaguchi, Suzuki, Do, Mizunoya, Nakamura, Sato, Furuse, Yamada, Ikeuchi, Tatsumi: "Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 84, Issue 11, pp. 744-50, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN409930) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody
Western Blotting (WB) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Western Blot: Integrin alpha M/CD11b Antibody [ABIN4099304] - Detection of CD11b in R...
Simple Western (SimWes) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Simple Western: Integrin alpha M/CD11b Antibody [ABIN4099304] - Simple Western lane v...
Immunofluorescence (IF) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunocytochemistry/Immunofluorescence: Integrin alpha M/CD11b Antibody [ABIN4099304]...
Immunofluorescence (IF) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunocytochemistry/Immunofluorescence: Integrin alpha M/CD11b Antibody [ABIN4099304]...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunohistochemistry-Frozen: CD11b Antibody - Blue: DAPI+ nuclei Red: CD11b Human lu...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunohistochemistry-Frozen: CD11b Antibody - Blue: DAPI+ nuclei. Red: CD11b. Lung c...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunohistochemistry-Frozen: CD11b Antibody - Mouse spleen. Image from verified cust...