anti-Non-Human Primate CD11b antibody for Flow Cytometry

Recommended CD11b Antibody

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Human, Non-Human Primate, Mouse (Murine), Rabbit
655
431
146
79
35
33
30
25
18
13
10
9
8
8
6
5
4
4
2
Host
Rat
475
345
175
16
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to APC
139
116
90
61
17
16
14
12
11
11
10
10
10
9
9
6
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
781
247
207
155
139
111
75
59
49
32
20
14
13
10
5
5
4
4
3
2
2
1
1
1
1
Options

Available images

Catalog No. ABIN1690733
$ 394.53
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
26.43473 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 ICRF44 4
26.43473 ABIN2749103 Func IHC (f) ICC FACS IP PE Mouse IgG1 ICRF44 4
22.594666 ABIN457369 FACS Func IHC (f) IP PE Rat IgG2b M1-70 9
22.594666 ABIN457346 FACS Func IHC (f) IP FITC Rat IgG2b M1-70 8
18.094666 ABIN457402 FACS Func IHC (f) IP Rat IgG2b M1-70 5
18.094666 ABIN1981858 Func IHC (f) ICC FACS IP Biotin Mouse IgG1 ICRF44 4
18.094666 ABIN1027692 FACS Func ICC IHC (f) IP Mouse IgG1 ICRF44 3
18.094666 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 ICRF44 3
13.594666 ABIN5633268 FACS Func IHC (f) IP Rat IgG2b M1-70 0

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Non-Human Primate, Mouse (Murine), Rabbit
(655), (431), (146), (79), (35), (33), (30), (25), (18), (13), (10), (9), (8), (8), (6), (5), (4), (4), (2)
Host Rat
(475), (345), (175), (16), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to APC
(139), (116), (90), (61), (17), (16), (14), (12), (11), (11), (10), (10), (10), (9), (9), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(781), (247), (207), (155), (139), (111), (75), (59), (49), (32), (20), (14), (13), (10), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 10 references available
Supplier

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1690733) Images
Specificity The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Cross-Reactivity (Details) Human, Non-Human Primates, Mouse, Rabbit
Immunogen B10 mouse spleen cells enriched for T cells
Clone M1-70
Isotype IgG2b
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN1690733) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN1690733) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis.
Comment

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN1690733) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD11b antibody (ABIN1690733)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Takagi, Numazaki, Kajiwara, Abe, Ishii, Kato, Kojima: "Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages." in: Glycobiology, Vol. 19, Issue 3, pp. 258-66, 2009

Leon, Contractor, Fuss, Marth, Lahey, Iwaki, la Sala, Hoffmann, Strober, Kelsall: "Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 10, pp. 6974-82, 2006

Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003

Zhang, McCormick, Desai, Wu, Gilliam: "Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 3088-98, 2002

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000

Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, 2000

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1690733) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (APC) (ABIN1690733) Surface staining of CD11b in murine peritoneal fluid with anti-CD11b (M1/70) APC.