anti-Non-Human Primate CD11b antibody for Immunocytochemistry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Human, Non-Human Primate
753
503
173
105
37
33
32
25
14
13
10
9
9
8
6
5
4
4
2
2
Host
Mouse
529
412
164
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to PE
144
123
97
62
19
17
16
16
14
14
14
13
12
11
9
9
9
8
8
7
6
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
921
272
221
202
173
163
93
92
91
61
26
21
13
12
9
8
5
5
4
3
3
2
1
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2749103
$ 366.85
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 Log in to see ICRF44 4
1 ABIN1981858 Func IHC (f) ICC FACS IP Biotin Mouse IgG1 Log in to see ICRF44 4
1 ABIN1027692 FACS Func ICC IHC (f) IP Mouse IgG1 Log in to see ICRF44 3
1 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 Log in to see ICRF44 3

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Non-Human Primate
(753), (503), (173), (105), (37), (33), (32), (25), (14), (13), (10), (9), (9), (8), (6), (5), (4), (4), (2), (2)
Host Mouse
(529), (412), (164), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to PE
(144), (123), (97), (62), (19), (17), (16), (16), (14), (14), (14), (13), (12), (11), (9), (9), (9), (8), (8), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
(921), (272), (221), (202), (173), (163), (93), (92), (91), (61), (26), (21), (13), (12), (9), (8), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN2749103) Images
Specificity The mouse monoclonal antibody ICRF44 recognizes CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
HLDA IV, WS Code M047
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen Rheumatoid synovial cells and human monocytes.
Clone ICRF44
Isotype IgG1
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN2749103) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN2749103) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN2749103) Images back to top
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD11b antibody (ABIN2749103)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Tieu, Lee, Sun, Lejeune, Recinos, Ju, Spratt, Guo, Milewicz, Tilton, Brasier: "An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice." in: The Journal of clinical investigation, Vol. 119, Issue 12, pp. 3637-51, 2009

Masten, Olson, Tarleton, Rund, Schuyler, Mehran, Archibeque, Lipscomb: "Characterization of myeloid and plasmacytoid dendritic cells in human lung." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7784-93, 2006

Hua, Sakamoto, Nagaoka: "Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils." in: Journal of leukocyte biology, Vol. 71, Issue 4, pp. 632-40, 2002

Sánchez-Torres, García-Romo, Cornejo-Cortés, Rivas-Carvalho, Sánchez-Schmitz: "CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells." in: International immunology, Vol. 13, Issue 12, pp. 1571-81, 2001

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN2749103) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (PE) (ABIN2749103) Surface staining of human peripheral blood with anti-CD11b (ICRF44) Alexa Fluor® 488.