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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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21.811565 | ABIN457369 | FACS Func IHC (f) IP | PE | Rat | IgG2b | Log in to see | M1-70 | 9 | ||
21.811565 | ABIN457346 | FACS Func IHC (f) IP | FITC | Rat | IgG2b | Log in to see | M1-70 | 8 | ||
20.311565 | ABIN2749102 | Func IHC (f) ICC FACS IP | FITC | Mouse | IgG1 | Log in to see | ICRF44 | 4 | ||
20.311565 | ABIN2749103 | Func IHC (f) ICC FACS IP | PE | Mouse | IgG1 | Log in to see | ICRF44 | 4 | ||
17.311565 | ABIN457402 | FACS Func IHC (f) IP | Rat | IgG2b | Log in to see | M1-70 | 5 | |||
17.311565 | ABIN1981858 | Func IHC (f) ICC FACS IP | Biotin | Mouse | IgG1 | Log in to see | ICRF44 | 4 | ||
17.311565 | ABIN1027692 | FACS Func ICC IHC (f) IP | Mouse | IgG1 | Log in to see | ICRF44 | 3 | |||
17.311565 | ABIN2749012 | Func IHC (f) ICC FACS IP | Mouse | IgG1 | Log in to see | ICRF44 | 3 | |||
12.811565 | ABIN5633268 | FACS Func IHC (f) IP | Rat | IgG2b | Log in to see | M1-70 | 0 |
General |
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Antigen | Integrin alpha M (ITGAM) Antibodies |
Reactivity | Human, Non-Human Primate, Mouse (Murine), Rabbit Alternatives |
Host | Rat Alternatives |
Clonality (Clone) | |
Conjugate | This CD11b antibody is conjugated to APC Alternatives |
Application |
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
Alternatives
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10 references available |
Supplier | Log in to see |
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1690733) Images |
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Specificity | The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions. |
Cross-Reactivity (Details) | Human, Non-Human Primates, Mouse, Rabbit |
Immunogen | B10 mouse spleen cells enriched for T cells |
Clone | M1-70 |
Isotype | IgG2b |
Plasmids, Primers & others | |
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN1690733) Images back to top |
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Antigen | |
Alternative Name | CD11b (ITGAM Antibody Abstract) |
Background | CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. |
Pathways | Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation |
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN1690733) Images back to top |
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Application Notes | The reagent is designed for Flow Cytometry analysis. |
Comment |
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
Restrictions | For Research Use only |
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN1690733) Images back to top |
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Concentration | 0.5 mg/mL |
Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice |
Do not freeze. Avoid prolonged exposure to light. |
Storage | 4 °C |
Storage Comment | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. |
References for anti-CD11b antibody (ABIN1690733)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top |
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Product cited in: |
Takagi, Numazaki, Kajiwara, Abe, Ishii, Kato, Kojima: "Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages." in: Glycobiology, Vol. 19, Issue 3, pp. 258-66, 2009 Leon, Contractor, Fuss, Marth, Lahey, Iwaki, la Sala, Hoffmann, Strober, Kelsall: "Antibodies to complement receptor 3 treat established inflammation in murine models of colitis and a novel model of psoriasiform dermatitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 10, pp. 6974-82, 2006 Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003 Zhang, McCormick, Desai, Wu, Gilliam: "Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 3088-98, 2002 Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000 Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, 2000 Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983 Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981 Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 |
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN1690733) back to top |
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Supplier Images |