anti-Rat (Rattus) CD11b antibody for Functional Studies

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Rat (Rattus)
763
506
175
109
38
34
32
25
15
13
10
10
9
8
6
5
4
4
2
2
Host
Mouse
539
417
161
17
5
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
146
127
97
64
19
17
17
16
14
14
14
13
12
11
9
9
9
8
8
7
6
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
937
276
225
200
170
168
91
91
90
60
26
21
13
13
9
8
5
5
4
3
3
2
1
1
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN120414
$ 742.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120412 Func FACS IHC (fro) IF IHC (p) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN120413 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN2478200 Func IHC (fro) FACS Mouse IgG1 Log in to see ED7 4

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Rat (Rattus)
(763), (506), (175), (109), (38), (34), (32), (25), (15), (13), (10), (10), (9), (8), (6), (5), (4), (4), (2), (2)
Host Mouse
(539), (417), (161), (17), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(146), (127), (97), (64), (19), (17), (17), (16), (14), (14), (14), (13), (12), (11), (9), (9), (9), (8), (8), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(937), (276), (225), (200), (170), (168), (91), (91), (90), (60), (26), (21), (13), (13), (9), (8), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN120414) Images
Purification Affinity chromatography on Protein G
Immunogen Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Clone OX-42
Isotype IgG2a
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN120414) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10116
UniProt Q63001
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN120414) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN120414) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD11b antibody (ABIN120414)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Milligan, Cunningham, Levitt: "Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain." in: The Journal of comparative neurology, Vol. 314, Issue 1, pp. 125-35, 1992

Robinson, White, Mason: "Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3." in: Immunology, Vol. 57, Issue 2, pp. 239-47, 1986

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN120414) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (ABIN120414) Staining of rat peritoneal macrophages with MOUSE ANTI RAT CD11b(ABIN120414).