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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN120413 | Func FACS IHC (fro) IHC (p) IP | Mouse | IgG2a | Log in to see | OX-42 | 4 | |||
1 | ABIN120412 | Func FACS IHC (fro) IF IHC (p) IP | Mouse | IgG2a | Log in to see | OX-42 | 4 | |||
1 | ABIN2478200 | Func IHC (fro) FACS | Mouse | IgG1 | Log in to see | ED7 | 4 |
General |
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Antigen | Integrin alpha M (ITGAM) Antibodies |
Reactivity | Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This CD11b antibody is un-conjugated Alternatives |
Application |
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
Alternatives
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4 references available |
Supplier | Log in to see |
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN120414) Images |
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Purification | Affinity chromatography on Protein G |
Immunogen | Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line. |
Clone | OX-42 |
Isotype | IgG2a |
Plasmids, Primers & others | |
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN120414) Images back to top |
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Antigen | |
Alternative Name | CD11b (ITGAM Antibody Abstract) |
Background | The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor |
Gene ID | 10116 |
UniProt | Q63001 |
Pathways | Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation |
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN120414) Images back to top |
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Application Notes |
Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN120414) Images back to top |
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Concentration | 1.0 mg/mL |
Buffer | PBS, pH 7.2 containing 0.09 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. |
References for anti-CD11b antibody (ABIN120414)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top |
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Background publications |
Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 Milligan, Cunningham, Levitt: "Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain." in: The Journal of comparative neurology, Vol. 314, Issue 1, pp. 125-35, 1992 Robinson, White, Mason: "Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3." in: Immunology, Vol. 57, Issue 2, pp. 239-47, 1986 Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 |
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN120414) back to top |
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Supplier Images |