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|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Epitope||Internal Region, AA 250-350 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
|31 references available|
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Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN409930) Images
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]|
|Plasmids, Primers & others|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN409930) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
|Background||Gene Symbol: ITGAM|
|Molecular Weight||Theoretical MW: 127 kDa|
|Pathways||Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN409930) Images back to top
|Application Notes||Western Blot 2 μg/mL, Simple Western 1:50, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry-Paraffin 1:400, Immunohistochemistry-FrozenThis CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN409930) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-CD11b antibody (ABIN409930)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
|Product cited in:||
Schmidt, Hobbing, Donninger, Clark: "RASSF1A Deficiency Enhances RAS-Driven Lung Tumorigenesis." in: Cancer research, Vol. 78, Issue 10, pp. 2614-2623, 2018
Periyasamy, Thangaraj, Guo, Hu, Callen, Buch: "Epigenetic Promoter DNA Methylation of miR-124 Promotes HIV-1 Tat-Mediated Microglial Activation via MECP2-STAT3 Axis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 38, Issue 23, pp. 5367-5383, 2018
Xu, Xu, Ge, Tian, Zhao, Guo: "Anti-inflammatory effect of low molecular weight fucoidan from Saccharina japonica on atherosclerosis in apoE-knockout mice." in: International journal of biological macromolecules, Vol. 118, Issue Pt A, pp. 365-374, 2018
Baranova, Souza, Bocharov, Vishnyakova, Hu, Vaisman, Amar, Chen, Remaley, Patterson, Yuen, Star, Eggerman: "Human SR-BII mediates SAA uptake and contributes to SAA pro-inflammatory signaling in vitro and in vivo." in: PLoS ONE, Vol. 12, Issue 4, pp. e0175824, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).
Li, Lu, Sheng, Zhu, Sun, Zhou, Yang, Xue, Chen, Tian, Du, Yan, Zhu, Xing, Li, Lin, Qiu, Su, Huang, Yan, Yin: "Hexokinase 2-dependent hyperglycolysis driving microglial activation contributes to ischemic brain injury." in: Journal of neurochemistry, Vol. 144, Issue 2, pp. 186-200, 2017 (Sample species: Rat (Rattus)).
Gao, Gülcüler, Golbach, Block, Zarbock, Martin-Villalba: "Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion." in: eLife, Vol. 5, 2017
Kim, Kang, Choi, Joo, Kim, Song: "Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-β2 induces anti-tumor immune activation." in: Oncotarget, Vol. 8, Issue 9, pp. 15858-15877, 2017
Algieri, Rodriguez-Nogales, Garrido-Mesa, Camuesco, Vezza, Garrido-Mesa, Utrilla, Rodriguez-Cabezas, Pischel, Galvez: "Intestinal anti-inflammatory activity of calcium pyruvate in the TNBS model of rat colitis: Comparison with ethyl pyruvate." in: Biochemical pharmacology, Vol. 103, pp. 53-63, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Makar, Nimmagadda, Singh, Lam, Mubariz, Judge, Trisler, Bever: "TrkB agonist, 7,8-dihydroxyflavone, reduces the clinical and pathological severity of a murine model of multiple sclerosis." in: Journal of neuroimmunology, Vol. 292, pp. 9-20, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Kim, Kim, Wu, Park, Ha, Kim, Lee, Lee, Kwon: "Extracellular Release of CD11b by TLR9 Stimulation in Macrophages." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150677, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Xu, Meng, Erben, Wang, Glauben, Kühl, Wu, Ma, Hu, Wang, Sun, Jia, Wu, Chen, Siegmund, Qin: "Myeloid-derived suppressor cells promote B-cell production of IgA in a TNFR2-dependent manner." in: Cellular & molecular immunology, 2016
Yang, Scott, Monickaraj, Xu, Ardekani, Nitta, Cabrera, McGuire, Mohideen, Das, Ghosh: "Basement membrane stiffening promotes retinal endothelial activation associated with diabetes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 2, pp. 601-11, 2016
Rawat, Spector: "Development and characterization of a human microglia cell model of HIV-1 infection." in: Journal of neurovirology, Vol. 23, Issue 1, pp. 33-46, 2016
Ulasov, Borovjagin, Kaverina, Schroeder, Shah, Lin, Baryshnikov, Cobbs: "MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo." in: Cancer letters, Vol. 365, Issue 2, pp. 240-50, 2015
Stiess, Wegehingel, Nguyen, Nickel, Bradke, Cambridge: "A Dual SILAC Proteomic Labeling Strategy for Quantifying Constitutive and Cell-Cell Induced Protein Secretion." in: Journal of proteome research, Vol. 14, Issue 8, pp. 3229-38, 2015 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence
Makar, Gerzanich, Nimmagadda, Jain, Lam, Mubariz, Trisler, Ivanova, Woo, Kwon, Bryan, Bever, Simard: "Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 12, pp. 210, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Sakaguchi, Shono, Suzuki, Sawano, Anderson, Do, Ohtsubo, Mizunoya, Sato, Nakamura, Furuse, Yamada, Ikeuchi, Tatsumi: "Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle." in: The international journal of biochemistry & cell biology, Vol. 54, pp. 272-85, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
Sawano, Suzuki, Do, Ohtsubo, Mizunoya, Ikeuchi, Tatsumi: "Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 85, Issue 12, pp. 994-1000, 2014
Gil-Pisa, Cebrián, Ortega, Meana, Sulzer: "Cytokine pathway disruption in a mouse model of schizophrenia induced by Munc18-1a overexpression in the brain." in: Journal of neuroinflammation, Vol. 11, pp. 128, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting
Shono, Sakaguchi, Suzuki, Do, Mizunoya, Nakamura, Sato, Furuse, Yamada, Ikeuchi, Tatsumi: "Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 84, Issue 11, pp. 744-50, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN409930) back to top