anti-Rat (Rattus) CD11b antibody for Immunoprecipitation

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Rat (Rattus)
762
502
178
98
37
33
31
25
18
14
10
10
9
9
6
5
4
4
2
2
Host
Mouse
534
401
176
17
5
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
143
125
99
65
17
17
16
15
14
13
12
12
11
11
11
9
7
7
7
7
6
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
905
272
221
201
184
162
94
90
89
62
21
20
13
13
8
8
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN234503
$ 517.73
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN2688912 BR IHC (fro) FACS IF IP Mouse IgG2a, kappa Log in to see OX 9
10 ABIN1177299 BR IHC (fro) FACS IF IP Mouse IgG2a, kappa Log in to see OX-42 7
10 ABIN2935006 IF/ICC IHC IP WB Rabbit AA 31-160 Log in to see Polyclonal 0
8.5 ABIN2478198 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4
7 ABIN2935007 IF/ICC IHC IP WB Rabbit AA 598-700 Log in to see Polyclonal 0
7 ABIN2935009 IF/ICC IHC IP WB Rabbit AA 870-1004 Log in to see Polyclonal 0
5.5 ABIN2478210 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4
4 ABIN120414 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4
4 ABIN2662974 Agon FACS IF IP PE Mouse IgG2a kappa Log in to see OX-42 6
1 ABIN120413 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN120412 Func FACS IHC (fro) IF IHC (p) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN182004 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN225690 BR FACS IHC IHC (fro) IP Rat IgG2b Log in to see M1-70 0
1 ABIN600700 FACS IHC IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN2222442 FACS IP IHC FITC Mouse IgG2a Log in to see 2Q913 0
1 ABIN217775 FACS IHC IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN210170 FACS IHC IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN2222425 FACS IP IHC Mouse IgG2a Log in to see 3H953 0
1 ABIN2222424 FACS IP IHC Mouse IgG2a Log in to see 3H953 0
1 ABIN2222441 FACS IP IHC Mouse IgG2a Log in to see 2Q913 0

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Rat (Rattus)
(762), (502), (178), (98), (37), (33), (31), (25), (18), (14), (10), (10), (9), (9), (6), (5), (4), (4), (2), (2)
Host Mouse
(534), (401), (176), (17), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(143), (125), (99), (65), (17), (17), (16), (15), (14), (13), (12), (12), (11), (11), (11), (9), (7), (7), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(905), (272), (221), (201), (184), (162), (94), (90), (89), (62), (21), (20), (13), (13), (8), (8), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN234503) Images
Specificity Recognizes most macrophages (including resident peritoneal and activated macrophages), kupffer cells, but only about 35% of alvoelar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 kDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3.
Characteristics Monoclonal antibody to Rat CD11b.
Purification Protein A Chromatography. Cell Culture. Tissue culture supernatant.
Immunogen Rat peritoneal macrophages
Clone OX-42
Isotype IgG2a
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN234503) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN234503) Images back to top
Application Notes Flow cytometry. Immunoprecipitation. Immunohistochemical studies of rat frozen tissue sections and paraffin embedded tissue sections following periodate-lysine-paraformaldehyde fixation. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN234503) Images back to top
Format Liquid
Buffer PBS, 10 mM Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Short-term (up to 1 month) store at 2-8 °C. Long term, aliquot and store at -28 °C.
Expiry Date 1 month

References for anti-CD11b antibody (ABIN234503)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Milligan, Cunningham, Levitt: "Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain." in: The Journal of comparative neurology, Vol. 314, Issue 1, pp. 125-35, 1992

Robinson, White, Mason: "Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3." in: Immunology, Vol. 57, Issue 2, pp. 239-47, 1986

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981