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General |
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Antigen | Clusterin (CLU) Antibodies |
Epitope | AA 488-501 Alternatives |
Reactivity | Human, Monkey Alternatives |
Host | Goat Alternatives |
Clonality | |
Conjugate | This Clusterin antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
Alternatives
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Product Details anti-Clusterin AntibodyTarget Details Clusterin Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Human CLU / Clusterin. This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1) |
Predicted Reactivity | Percent identity by BLAST analysis: Human, Gorilla, Marmoset (100%) Gibbon, Dog (93%) Monkey, Hamster, Rabbit, Horse (86%). |
Purification | Immunoaffinity purified |
Immunogen |
Synthetic peptide C-EKALQEYRKKHREE from the C-terminus of human CLU / Clusterin (NP_001822.2, NP_976084.1). Percent identity by BLAST analysis: Human, Gorilla, Marmoset (100%), Gibbon, Dog (93%), Monkey, Hamster, Rabbit, Horse (86%). Type of Immunogen: Synthetic peptide |
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Antigen | |
Alternative Name | CLU / Clusterin (CLU Antibody Abstract) |
Background |
Name/Gene ID: CLU Synonyms: CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Complement lysis inhibitor, CLI, AAG4, Aging-associated protein 4, Ku70-binding protein 1, KUB1, SGP2, APOJ, SGP-2, Sulfated glycoprotein 2, CLU2, Complement cytolysis inhibitor, NA1/NA2, SP-40 |
Gene ID | 1191 |
NCBI Accession | NP_001822, NP_976084 |
UniProt | P10909 |
Pathways | Apoptosis, Negative Regulation of intrinsic apoptotic Signaling |
Application DetailsProduct Details anti-Clusterin Antibody Target Details Clusterin Handling Images back to top |
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Application Notes |
Approved: ELISA (1:32000), IHC-P (2.5 μg/mL), WB (1:32000, 0.3 - 1 μg/mL) Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. |
Restrictions | For Research Use only |
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Format | Liquid |
Concentration | Lot specific |
Buffer | Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Minimize freezing and thawing. |
Storage | -20 °C |
Storage Comment | Store at -20°C. Minimize freezing and thawing. |
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Supplier Images |