anti-Bird (Avian) Cytochrome C antibody for Immunofluorescence

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • CYC
  • HCS
  • THC4
  • cyct
  • cyc
  • hcs
  • CYCS
  • cytc
  • CYCSA
  • CG17903
  • Cyt C
  • Cyt c
  • Cyt-c2
  • CytC-2
  • Cytc
  • Cytc-p
  • DC4
  • DMc01
  • Dmel\\CG17903
  • cyt c
  • cyt c-p
  • cyt-c-p
  • cyt-c2
  • cyt.c
  • cytochrome c
  • dc4
  • pDMc01
  • wu:fk52b01
  • zgc:86706
  • cytochrome c, somatic
  • cytochrome c, somatic S homeolog
  • Cytochrome c proximal
  • cytochrome c, somatic b
  • cytochrome c-like
  • cytochrome c
  • CYCS
  • cycs.S
  • cycs
  • Cycs
  • Cyt-c-p
  • cycsb
  • LOC100724240
  • CYC
  • LOC100847700
  • LOC101107954
  • LOC106829744
  • LOC106992390
Epitope
AA 93-104
26
16
14
14
8
7
6
5
4
4
4
3
3
3
2
1
1
1
1
Reactivity
Amphibian, Bird (Avian), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
405
341
250
113
89
77
43
38
31
29
25
25
24
20
12
10
8
8
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
298
158
7
3
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
21
14
13
8
8
8
7
7
6
6
6
5
5
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
348
194
150
114
105
68
50
42
36
26
22
13
6
5
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN4301975
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4301988 CyTOF FACS IF IHC (p) WB Mouse IgG2b kappa AA 93-104 Log in to see SPM389 0
1 ABIN4301978 FACS IF IHC (p) WB Mouse IgG2b kappa Log in to see CTC05 0
1 ABIN5948579 FACS IF IHC IHC (p) Janelia Fluor® 549 Mouse IgG2b kappa AA 93-104 Log in to see SPM389 0
1 ABIN5948580 FACS IF IHC IHC (p) Janelia Fluor® 646 Mouse IgG2b kappa AA 93-104 Log in to see SPM389 0

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 93-104
(26), (16), (14), (14), (8), (7), (6), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Amphibian, Bird (Avian), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(405), (341), (250), (113), (89), (77), (43), (38), (31), (29), (25), (25), (24), (20), (12), (10), (8), (8), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(298), (158), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(21), (14), (13), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(348), (194), (150), (114), (105), (68), (50), (42), (36), (26), (22), (13), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Specificity It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Purification Protein A or G purified
Immunogen Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
Clone SPM389
Isotype IgG2b kappa
Plasmids, Primers & others

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background Gene Symbol: CYCS
Molecular Weight Theoretical MW: 15 kDa
Gene ID 54205
UniProt P99999
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Western Blot 0.5 - 1.0 μg/mL, Flow Cytometry 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 μg/mL, Immunofluorescence 0.5 - 1.0 μg/mLHu-chromosome location: 7p15.3 Immunohistochemistry-Paraffin 0.25 - 0.5 μg/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Format Liquid
Buffer 1.0 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.