anti-Dog (Canine) Cytochrome C antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • CYC
  • HCS
  • THC4
  • cyct
  • cyc
  • hcs
  • CYCS
  • cytc
  • CYCSA
  • CG17903
  • Cyt C
  • Cyt c
  • Cyt-c2
  • CytC-2
  • Cytc
  • Cytc-p
  • DC4
  • DMc01
  • Dmel\\CG17903
  • cyt c
  • cyt c-p
  • cyt-c-p
  • cyt-c2
  • cyt.c
  • cytochrome c
  • dc4
  • pDMc01
  • wu:fk52b01
  • zgc:86706
  • cytochrome c, somatic
  • cytochrome c, somatic S homeolog
  • Cytochrome c proximal
  • cytochrome c, somatic b
  • cytochrome c-like
  • cytochrome c
  • CYCS
  • cycs.S
  • cycs
  • Cycs
  • Cyt-c-p
  • cycsb
  • LOC100724240
  • CYC
  • LOC100847700
  • LOC101107954
  • LOC106829744
  • LOC106992390
Reactivity
Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
393
329
236
115
93
81
46
38
33
31
25
25
24
20
12
11
8
8
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
281
163
7
3
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is conjugated to CF®640R
20
12
12
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
353
176
148
123
115
73
49
41
40
26
23
13
6
5
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6170388
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6170390 FACS IF IHC (f) WB CF®543 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170398 FACS IF IHC (f) WB CF®568 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170406 FACS IF IHC (f) WB CF®488A Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170386 FACS IF IHC (f) WB CF405M Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170394 FACS IF IHC (f) WB CF®555 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170400 FACS IF IHC (f) WB CF®770 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170404 FACS IF IHC (f) WB CF®680R Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170384 FACS IF IHC (f) WB CF®405S Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170408 FACS IF IHC (f) WB CF®594 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170392 FACS IF IHC (f) WB CF®647 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170396 FACS IF IHC (f) WB CF®660R Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170402 FACS IF IHC (f) WB CF®680 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170419 FACS IF IHC (f) WB Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170410 FACS IF IHC (f) WB APC Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170418 FACS IF IHC (f) WB PE Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170413 FACS IF IHC (f) WB Biotin Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170417 FACS IF IHC (f) WB PerCP Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170411 FACS IF IHC (f) WB Alkaline Phosphatase (AP) Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170421 FACS IF IHC (f) WB Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170415 FACS IF IHC (f) WB HRP Mouse IgG2b, kappa Log in to see CTC05 0

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Reactivity Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
(393), (329), (236), (115), (93), (81), (46), (38), (33), (31), (25), (25), (24), (20), (12), (11), (8), (8), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(281), (163), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is conjugated to CF®640R
(20), (12), (12), (7), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(353), (176), (148), (123), (115), (73), (49), (41), (40), (26), (23), (13), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Purpose Mouse Monoclonal anti-Cytochrome-C (CTC05), CF640R Conjugate
Characteristics Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant cytochrome c protein
Clone CTC05
Isotype IgG2b, kappa
Plasmids, Primers & others

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome-C (CYCS Antibody Abstract)
Background CYC, CYCS, HCS, THC4
Molecular Weight 15 kDa
Gene ID 54205, 437060
UniProt P99999
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light