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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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4 | ABIN187764 | IHC (fro) WB | Sheep | Log in to see | Polyclonal | 0 | ||||
1 | ABIN264906 | IHC (fro) IP WB | Sheep | IgG | Log in to see | Polyclonal | 0 |
General |
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Antigen | Cytochrome C, Somatic (CYCS) Antibodies |
Reactivity | Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mammalian, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This Cytochrome C antibody is conjugated to Biotin Alternatives |
Application |
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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32 references available |
Supplier | Log in to see |
Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling References for anti-Cytochrome C antibody (ABIN251788) Images |
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Specificity | An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached). |
Purification | Protein G purified |
Immunogen | Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991). |
Clone | 7H8-2C12 |
Isotype | IgG2b kappa |
Plasmids, Primers & others | |
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C antibody (ABIN251788) Images back to top |
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Antigen | |
Alternative Name | Cytochrome C (CYCS Antibody Abstract) |
Background | Gene Symbol: CYCS |
Gene ID | 54205 |
Pathways | Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity |
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C antibody (ABIN251788) Images back to top |
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Application Notes | Western Blot 0.05-0.5 μg/mL, Immunohistochemistry, Immunohistochemistry-Frozen 1:10-1:500Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C antibody (ABIN251788) Images back to top |
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Format | Liquid |
Buffer |
PBS Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Do not freeze. |
Storage | 4 °C |
Storage Comment | Store at 4°C. Do not freeze. |
References for anti-Cytochrome C antibody (ABIN251788)Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top |
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Product cited in: |
Csak, Dolganiuc, Kodys, Nath, Petrasek, Bala, Lippai, Szabo: "Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice." in: Hepatology (Baltimore, Md.), Vol. 53, Issue 6, pp. 1917-31, 2011 Kamal, Sreekanth, Kumar, Shankaraiah, Balakishan, Ramaiah, Pushpavalli, Ray, Bhadra: "Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines." in: European journal of medicinal chemistry, Vol. 45, Issue 6, pp. 2173-81, 2010 Douglas, Ryu, Kanaan, Del Carmen Rivero, Dugan, Haddad, Ali: "Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction." in: American journal of physiology. Cell physiology, Vol. 298, Issue 6, pp. C1594-602, 2010 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence Zall, Weber, Besch, Zantl, Häcker: "Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1." in: Molecular cancer, Vol. 9, pp. 164, 2010 Kumar, Coltas, Kumar, Chepeha, Bradford, Polverini: "Bcl-2 protects endothelial cells against gamma-radiation via a Raf-MEK-ERK-survivin signaling pathway that is independent of cytochrome c release." in: Cancer research, Vol. 67, Issue 3, pp. 1193-202, 2007 Zhu, Xu, Wang, Shibata, Uchiyama, Blomgren, Hagberg: "Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia." in: Journal of neurochemistry, Vol. 96, Issue 4, pp. 1016-27, 2006 Yamasaki, Wada, Kumatori, Nakagawa, Funao, Nakayama, Hisatsune, Kimura, Moss, Hirayama: "Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 11250-9, 2006 Meyer, Karawajew, Schrappe, Ludwig, Debatin, Stahnke: "Cytochrome c-related caspase-3 activation determines treatment response and relapse in childhood precursor B-cell ALL." in: Blood, Vol. 107, Issue 11, pp. 4524-31, 2006 Chen, Gan, Remold: "A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3707-16, 2006 (Sample species: Human). Further details: Flow Cytometry,Immunochromatography De Chiara, Marcocci, Torcia, Lucibello, Rosini, Bonini, Higashimoto, Damonte, Armirotti, Amodei, Palamara, Russo, Garaci, Cozzolino: "Bcl-2 Phosphorylation by p38 MAPK: identification of target sites and biologic consequences." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 21353-61, 2006 Boden, Homko, Mozzoli, Showe, Nichols, Cheung: "Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients." in: Diabetes, Vol. 54, Issue 3, pp. 880-5, 2005 Arnoult, Grodet, Lee, Estaquier, Blackstone: "Release of OPA1 during apoptosis participates in the rapid and complete release of cytochrome c and subsequent mitochondrial fragmentation." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35742-50, 2005 Wong, Chen, Chan, Chan, Chan, Chui, Fung, Waye, Tsui, Chan: "In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila." in: Biochemical and biophysical research communications, Vol. 337, Issue 2, pp. 720-9, 2005 (Sample species: Fruit Fly (Drosophila melanogaster)). Further details: Western Blotting Liang, Eid, Lewis, Kumar: "Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli." in: Cellular signalling, Vol. 17, Issue 2, pp. 243-51, 2004 Liu, Lee, Hong, Wei: "Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis." in: Annals of the New York Academy of Sciences, Vol. 1011, pp. 133-45, 2004 (Sample species: Human). Colell, García-Ruiz, Lluis, Coll, Mari, Fernández-Checa: "Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33928-35, 2003 (Sample species: Rat (Rattus)). Further details: Western Blotting Zhu, Wang, Cheng, Qiu, Xu, Simbruner, Blomgren: "Post-ischemic hypothermia-induced tissue protection and diminished apoptosis after neonatal cerebral hypoxia-ischemia." in: Brain research, Vol. 996, Issue 1, pp. 67-75, 2003 (Sample species: Rat (Rattus)). Further details: Western Blotting Bitzer, Armeanu, Prinz, Ungerechts, Wybranietz, Spiegel, Bernlöhr, Cecconi, Gregor, Neubert, Schulze-Osthoff, Lauer: "Caspase-8 and Apaf-1-independent caspase-9 activation in Sendai virus-infected cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29817-24, 2002 Chandra, Liu, Tang: "Early mitochondrial activation and cytochrome c up-regulation during apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50842-54, 2002 Narvaez, Welsh: "Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9101-7, 2001 Further details: Western Blotting |
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