anti-Frog Cytochrome C antibody for Western Blotting

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • CYC
  • HCS
  • THC4
  • cyct
  • cyc
  • hcs
  • CYCS
  • cytc
  • CYCSA
  • CG17903
  • Cyt C
  • Cyt c
  • Cyt-c2
  • CytC-2
  • Cytc
  • Cytc-p
  • DC4
  • DMc01
  • Dmel\\CG17903
  • cyt c
  • cyt c-p
  • cyt-c-p
  • cyt-c2
  • cyt.c
  • cytochrome c
  • dc4
  • pDMc01
  • wu:fk52b01
  • zgc:86706
  • cytochrome c, somatic
  • cytochrome c, somatic S homeolog
  • Cytochrome c proximal
  • cytochrome c, somatic b
  • cytochrome c-like
  • cytochrome c
  • CYCS
  • cycs.S
  • cycs
  • Cycs
  • Cyt-c-p
  • cycsb
  • LOC100724240
  • CYC
  • LOC100847700
  • LOC101107954
  • LOC106829744
  • LOC106992390
Epitope
AA 1-80
27
17
16
12
11
8
6
5
5
4
4
3
3
3
2
2
1
1
1
1
Reactivity
Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
393
329
236
115
93
81
46
38
33
31
25
25
24
20
12
11
8
8
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
281
163
7
3
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
20
12
12
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
353
176
147
123
115
73
49
42
40
26
23
13
6
5
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN3025122
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN3024593 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 93-104 Log in to see SPM389 0
4 ABIN3024590 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170388 FACS IF IHC (f) WB CF®640R Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170390 FACS IF IHC (f) WB CF®543 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170398 FACS IF IHC (f) WB CF®568 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170406 FACS IF IHC (f) WB CF®488A Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170386 FACS IF IHC (f) WB CF405M Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170394 FACS IF IHC (f) WB CF®555 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170400 FACS IF IHC (f) WB CF®770 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170404 FACS IF IHC (f) WB CF®680R Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170384 FACS IF IHC (f) WB CF®405S Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170408 FACS IF IHC (f) WB CF®594 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170392 FACS IF IHC (f) WB CF®647 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170396 FACS IF IHC (f) WB CF®660R Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170402 FACS IF IHC (f) WB CF®680 Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170419 FACS IF IHC (f) WB Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170410 FACS IF IHC (f) WB APC Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170418 FACS IF IHC (f) WB PE Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170413 FACS IF IHC (f) WB Biotin Mouse IgG2b, kappa Log in to see CTC05 0
1 ABIN6170417 FACS IF IHC (f) WB PerCP Mouse IgG2b, kappa Log in to see CTC05 0

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 1-80
(27), (17), (16), (12), (11), (8), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
(393), (329), (236), (115), (93), (81), (46), (38), (33), (31), (25), (25), (24), (20), (12), (11), (8), (8), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(281), (163), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(20), (12), (12), (7), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(353), (176), (147), (123), (115), (73), (49), (42), (40), (26), (23), (13), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Characteristics It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Purification Protein G affinity chromatography
Immunogen Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.
Clone 7H8-2C12
Isotype IgG2b, kappa
Plasmids, Primers & others

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Cytochrome C, Somatic (CYCS) (AA 1-80) antibody (ABIN3025122) Formalin-fixed, paraffin-embedded human pancreas stained with Cytochrome C antibody (...
Immunohistochemistry (IHC) image for anti-Cytochrome C, Somatic (CYCS) (AA 1-80) antibody (ABIN3025122) Formalin-fixed, paraffin-embedded human salivary tumor stained with Cytochrome C anti...