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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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4 | ABIN207924 | IHC IHC (fro) IP Neut | Mouse | IgG1 | Log in to see | 6H2-B4 | 0 | |||
2.1583147 | ABIN4301951 | BP IHC (fro) IP Neut | Mouse | IgG1 kappa | Log in to see | 6H2-B4 | 6 | |||
1 | ABIN2666072 | Neut | Mouse | IgG1 kappa | Log in to see | 6H2-B4 | 10 | |||
-12.841685 | ABIN4301950 | BP IHC (fro) IP Neut | Mouse | IgG1 kappa | Log in to see | 6H2-B4 | 0 |
General |
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Antigen | Cytochrome C, Somatic (CYCS) Antibodies |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This Cytochrome C antibody is un-conjugated Alternatives |
Application |
Neutralization (Neut)
Alternatives
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10 references available |
Supplier | Log in to see |
Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling References for anti-Cytochrome C antibody (ABIN2666071) Images |
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Purification | The antibody was purified by affinity chromatography. |
Clone | 6H2-B4 |
Isotype | IgG1 kappa |
Plasmids, Primers & others | |
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C antibody (ABIN2666071) Images back to top |
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Antigen | |
Alternative Name | Cytochrome C (CYCS Antibody Abstract) |
Background | Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain, the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining. |
Pathways | Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity |
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C antibody (ABIN2666071) Images back to top |
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Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C antibody (ABIN2666071) Images back to top |
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Concentration | 0.5 mg/mL |
Buffer | phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C |
Storage Comment | Upon receipt, store between 2°C and 8°C. |
References for anti-Cytochrome C antibody (ABIN2666071)Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top |
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Background publications |
McGuire, Barlan, Griffin, Wiethoff: "Adenovirus type 5 rupture of lysosomes leads to cathepsin B-dependent mitochondrial stress and production of reactive oxygen species." in: Journal of virology, Vol. 85, Issue 20, pp. 10806-13, 2011 She, Zhang, Marchionini, Diaz, Jetton, Kimball, Vary, Lang, Lynch: "Molecular characterization of skeletal muscle atrophy in the R6/2 mouse model of Huntington's disease." in: American journal of physiology. Endocrinology and metabolism, Vol. 301, Issue 1, pp. E49-61, 2011 Ferguson, Marietta, Van Den Berg: "UV-induced apoptosis is mediated independent of caspase-9 in MCF-7 cells: a model for cytochrome c resistance." in: The Journal of biological chemistry, Vol. 278, Issue 46, pp. 45793-800, 2003 Zhang, Gerstein: "The human genome has 49 cytochrome c pseudogenes, including a relic of a primordial gene that still functions in mouse." in: Gene, Vol. 312, pp. 61-72, 2003 Semenkova, Dudich, Dudich, Tokhtamisheva, Tatulov, Okruzhnov, Garcia-Foncillas, Palop-Cubillo, Korpela: "Alpha-fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation." in: European journal of biochemistry / FEBS, Vol. 270, Issue 21, pp. 4388-99, 2003 Chandra, Liu, Tang: "Early mitochondrial activation and cytochrome c up-regulation during apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50842-54, 2002 Shih, Wu, Hung, Yang, Lin: "Abrin triggers cell death by inactivating a thiol-specific antioxidant protein." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21870-7, 2001 Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996 Goshorn, Retzel, Jemmerson: "Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c." in: The Journal of biological chemistry, Vol. 266, Issue 4, pp. 2134-42, 1991 Jemmerson, Johnson, Burrell, Taylor, Jenkins: "A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells." in: European journal of immunology, Vol. 21, Issue 1, pp. 143-51, 1991 |
ImagesProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling References for anti-Cytochrome C antibody (ABIN2666071) back to top |
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Supplier Images |