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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN2793980 | ICC IHC (p) WB | Mouse | IgG2b | AA 1-80 | Log in to see | 7H8-2C12 | 0 | ||
-9.841685 | ABIN4301970 | FACS ICC IF IHC IHC (p) WB | Alexa Fluor 488 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301945 | FACS ICC IF IHC IHC (p) WB | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | |||
-12.841685 | ABIN4301960 | FACS ICC IF IHC IHC (p) WB | PerCP | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301972 | FACS ICC IF IHC IHC (p) WB | Alexa Fluor 700 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301956 | FACS ICC IF IHC IHC (p) WB | DyLight 680 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301959 | FACS ICC IF IHC IHC (p) WB | DyLight 755 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301957 | FACS ICC IF IHC IHC (p) WB | DyLight 488 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301962 | FACS ICC IF IHC IHC (p) WB | DyLight 550 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301964 | FACS ICC IF IHC IHC (p) WB | DyLight 405 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301955 | FACS ICC IF IHC IHC (p) WB | DyLight 650 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301963 | FACS ICC IF IHC IHC (p) WB | DyLight 350 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301969 | FACS ICC IF IHC IHC (p) WB | Alexa Fluor 405 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4301971 | FACS ICC IF IHC IHC (p) WB | Alexa Fluor 647 | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 | ||
-12.841685 | ABIN4995445 | FACS ICC IF IHC (p) | FITC | Mouse | IgG2b kappa | Log in to see | 7H8-2C12 | 0 |
General |
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Antigen | Cytochrome C, Somatic (CYCS) Antibodies |
Reactivity | Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mammalian, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This Cytochrome C antibody is un-conjugated Alternatives |
Application |
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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45 references available |
Supplier | Log in to see |
Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling References for anti-Cytochrome C antibody (ABIN252468) Images |
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Specificity | An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached). |
Purification | Protein G purified |
Immunogen | Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991). |
Clone | 7H8-2C12 |
Isotype | IgG2b kappa |
Plasmids, Primers & others | |
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C antibody (ABIN252468) Images back to top |
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Antigen | |
Alternative Name | Cytochrome C (CYCS Antibody Abstract) |
Background | Gene Symbol: CYCS |
Gene ID | 54205 |
UniProt | P99999 |
Pathways | Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity |
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C antibody (ABIN252468) Images back to top |
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Application Notes | Western Blot 0.05-0.5 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C antibody (ABIN252468) Images back to top |
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Format | Liquid |
Concentration | 0.5 mg/mL |
Buffer |
PBS containing 0.5 % BSA Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C,-20 °C |
Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
References for anti-Cytochrome C antibody (ABIN252468)Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top |
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Product cited in: |
Li, Zhao, Cheng, Rao, Yu, Ruan, Yuan, Yao: "Oxidative damage and mitochondrial injuries differ following pneumoperitoneum pressure in rabbit models of varying degrees of hydronephrosis." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6819-6827, 2018 Huang, Huang, Gou, Wang, Liao, Wang: "Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action." in: Bioconjugate chemistry, Vol. 27, Issue 9, pp. 2132-48, 2016 (Sample species: Human). Further details: Western Blotting Patel, Garikapati, Ramaiah, Polavarapu, Bhadra, Bhadra: "miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1." in: Life sciences, Vol. 164, pp. 60-70, 2016 (Sample species: Human). Further details: Western Blotting Waghela, Sharma, Dhumale, Pandey, Pathak: "Curcumin conjugated with PLGA potentiates sustainability, anti-proliferative activity and apoptosis in human colon carcinoma cells." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117526, 2015 (Sample species: Human). Further details: Western Blotting Dhumale, Waghela, Pathak: "Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells." in: IUBMB life, Vol. 67, Issue 5, pp. 361-73, 2015 (Sample species: Human). Further details: Western Blotting Pradhan, Pratheek, Garai, Kumar, Meena, Ghosh, Singh, Kumari, Chandrashekar, Goswami, Chattopadhyay, Kar, Maiti: "Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells." in: Cell biology international, Vol. 38, Issue 10, pp. 1118-31, 2014 Further details: Western Blotting Wang, Schilling, Niesman, Headrick, Finley, Kwan, Patel, Head, Roth, Yue, Patel: "Cardioprotective trafficking of caveolin to mitochondria is Gi-protein dependent." in: Anesthesiology, Vol. 121, Issue 3, pp. 538-48, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting Nogami, Takahama, Okushima, Oyoshi, Fujimoto, Inouye: "Tailor-made designer helical peptides that induce mitochondrion-mediated cell death without necrosis." in: Chembiochem : a European journal of chemical biology, Vol. 15, Issue 17, pp. 2571-6, 2014 (Sample species: Human). Further details: Western Blotting Kumar, Rao, Pal, Pal: "Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells." in: The international journal of biochemistry & cell biology, Vol. 53, pp. 302-19, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting Sagar, Kumar, Behera, Pal: "Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells." in: Journal of hazardous materials, Vol. 278, pp. 236-49, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting Zhang, Wang, Chen: "Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells." in: Cellular signalling, Vol. 24, Issue 5, pp. 1037-46, 2012 (Sample species: Human). Further details: Western Blotting Csak, Dolganiuc, Kodys, Nath, Petrasek, Bala, Lippai, Szabo: "Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice." in: Hepatology (Baltimore, Md.), Vol. 53, Issue 6, pp. 1917-31, 2011 Further details: Western Blotting Kamal, Sreekanth, Kumar, Shankaraiah, Balakishan, Ramaiah, Pushpavalli, Ray, Bhadra: "Synthesis and potential cytotoxic activity of new phenanthrylphenol-pyrrolobenzodiazepines." in: European journal of medicinal chemistry, Vol. 45, Issue 6, pp. 2173-81, 2010 Douglas, Ryu, Kanaan, Del Carmen Rivero, Dugan, Haddad, Ali: "Neuronal death during combined intermittent hypoxia/hypercapnia is due to mitochondrial dysfunction." in: American journal of physiology. Cell physiology, Vol. 298, Issue 6, pp. C1594-602, 2010 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence Zall, Weber, Besch, Zantl, Häcker: "Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1." in: Molecular cancer, Vol. 9, pp. 164, 2010 Further details: Western Blotting Kumar, Coltas, Kumar, Chepeha, Bradford, Polverini: "Bcl-2 protects endothelial cells against gamma-radiation via a Raf-MEK-ERK-survivin signaling pathway that is independent of cytochrome c release." in: Cancer research, Vol. 67, Issue 3, pp. 1193-202, 2007 Further details: Flow Cytometry,Immunochromatography Zhu, Xu, Wang, Shibata, Uchiyama, Blomgren, Hagberg: "Different apoptotic mechanisms are activated in male and female brains after neonatal hypoxia-ischaemia." in: Journal of neurochemistry, Vol. 96, Issue 4, pp. 1016-27, 2006 (Sample species: Mouse (Murine)). Further details: Western Blotting Yamasaki, Wada, Kumatori, Nakagawa, Funao, Nakayama, Hisatsune, Kimura, Moss, Hirayama: "Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 11250-9, 2006 Further details: Immunocytochemistry,Immunofluorescence Meyer, Karawajew, Schrappe, Ludwig, Debatin, Stahnke: "Cytochrome c-related caspase-3 activation determines treatment response and relapse in childhood precursor B-cell ALL." in: Blood, Vol. 107, Issue 11, pp. 4524-31, 2006 (Sample species: Human). Further details: Cell Sorting,Flow Cytometry,Immunochromatography Chen, Gan, Remold: "A mechanism of virulence: virulent Mycobacterium tuberculosis strain H37Rv, but not attenuated H37Ra, causes significant mitochondrial inner membrane disruption in macrophages leading to necrosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3707-16, 2006 (Sample species: Human). Further details: Flow Cytometry,Immunochromatography |
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