|Antigen||Cytochrome C, Somatic (CYCS) Antibodies|
|Reactivity||Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This Cytochrome C antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
|1 reference available|
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Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling References for anti-Cytochrome C antibody (ABIN317119) Images
|Specificity||This antiobdy recognises cytochrome c, originally termed Apaf-2, an evolutionarily conserved protein which acts as an essential mitochondrial electron carrier between respirable substrates and oxygen, during aerobic energy production. Clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9. Removal of sodium azide is recommended prior to use in functional assays|
|Characteristics||Synonyms: CYCS, CYC|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Human cytochrome c|
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C antibody (ABIN317119) Images back to top
|Alternative Name||Cytochrome C (CYCS Antibody Abstract)|
|Background||A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3).Synonyms: CYC, CYCS|
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C antibody (ABIN317119) Images back to top
Flow Cytometry. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C antibody (ABIN317119) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
|Expiry Date||12 months|
References for anti-Cytochrome C antibody (ABIN317119)Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top
Neame, Rubin, Philpott: "Blocking cytochrome c activity within intact neurons inhibits apoptosis." in: The Journal of cell biology, Vol. 142, Issue 6, pp. 1583-93, 1998