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General |
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Antigen | Cytochrome C, Somatic (CYCS) Antibodies |
Reactivity | Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Kangaroo, Monkey, Mouse (Murine), Pig (Porcine), Pigeon, Rabbit, Rat (Rattus), Sheep (Ovine), Snail, Tunafish, Turkey, Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This Cytochrome C antibody is un-conjugated Alternatives |
Application |
Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling Images |
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Blocking Peptide | Blocking peptide for this product available: ABIN1004318 |
Specificity | The cytochrome c antibody detects the 12.6 kDa cytochrome c from human,mouse,and rat samples. |
Purification | affinity purified |
Immunogen | Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c (ID BV-52). |
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | Cytochrome c (CYCS Antibody Abstract) |
Background | Cytochrome c (MW 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and Caspase-9 to activate Caspase-3 and other caspases. |
Pathways | Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity |
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top |
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Application Notes | The antibody can be used for Western blotting (0.5-4 µg/mL) and immunoprecipitation (10-20 µg/mL). However, the optimal conditions should be determined individually. |
Restrictions | For Research Use only |
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top |
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Format | Liquid |
Concentration | 0.2 mg/mL |
Buffer | PBS containing 30 % glycerol, 0.0 % BSA, and 0.00 % thimerosal. |
Preservative | Thimerosal (Merthiolate) |
Precaution of Use | Biohazard Informations: This product contains thimerosal which is hazardous. |
Handling Advice |
Can be diluted in other aqueous buffers at the concentrations determined for the respective application. Avoid repeated freeze/defrost cycles. |
Storage | -20 °C |
Storage Comment | For long-term storage, aliquot and freeze at -70 °C. |
Expiry Date | 12 months |