anti-Human DFFB antibody for Western Blotting

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor subunit beta
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
Middle Region
19
18
15
8
5
5
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
127
77
60
Host
Rabbit
129
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
128
38
26
26
21
21
18
10
5
3
1
1
Options
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Catalog No. ABIN2784747
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.280567 ABIN2707475 WB Rabbit N-Term Log in to see Polyclonal 0
3.280567 ABIN4892030 WB Rabbit Isoform beta Log in to see Polyclonal 1
3.280567 ABIN951896 FACS IHC (p) WB Rabbit Ig Fraction AA 1-30, N-Term Log in to see Polyclonal 4
3.280567 ABIN967293 WB Rabbit Ig Fraction AA 203-218 Log in to see Polyclonal 4
3.280567 ABIN266063 WB Mouse IgG Log in to see 36A349 3
3.280567 ABIN653798 FACS IHC (p) WB Rabbit Ig Fraction AA 1-30, N-Term Log in to see Polyclonal 1
3.280567 ABIN4305065 ELISA ICC IF WB Rabbit Center Log in to see Polyclonal 0
3.280567 ABIN4305068 ELISA ICC IF WB Rabbit Log in to see Polyclonal 0
3.280567 ABIN1858217 ICC IHC WB Rabbit IgG AA 87-323 Log in to see Polyclonal 0
3.280567 ABIN1030918 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal 0
3.280567 ABIN1031351 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
3.280567 ABIN1030919 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal 0
3.280567 ABIN514957 WB Rabbit AA 1-338, full length Log in to see Polyclonal 0
3.280567 ABIN560596 ELISA WB Mouse AA 229-338, partial Log in to see Polyclonal 0
3.280567 ABIN4305066 ELISA ICC IF WB Rabbit N-Term Log in to see Polyclonal 0
3.280567 ABIN4903473 WB Rabbit IgG Log in to see Polyclonal 0
3.280567 ABIN634501 WB Rabbit Log in to see Polyclonal 0
3.280567 ABIN189899 WB Rabbit AA 203-218 Log in to see Polyclonal 0
3.280567 ABIN189898 WB Rabbit AA 3-18 Log in to see Polyclonal 0
3.280567 ABIN189883 WB Rabbit AA 147-164 Log in to see Polyclonal 0

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope Middle Region
(19), (18), (15), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(127), (77), (60)
Host Rabbit
(129), (29)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(128), (38), (26), (26), (21), (21), (18), (10), (5), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN2784747) Images
Blocking Peptide Blocking peptide for this product available: ABIN975736
Sequence EYFYGLLFTS ENLKLVHIVC HKKTTHKLNC DPSRIYKPQT RLKRKQPVRK
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against DFFB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human DFFB

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB antibody (ABIN2784747) Images back to top
Antigen
Alternative Name DFFB (DFFB Antibody Abstract)
Background DFFB is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. DFFB degrades naked DNA and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAD, CPAN, DFF-40, DFF2, DFF40
Protein Interaction Partner: DFFA, UBC, NUBP1, MARCKS, APP, CAND1, COPS5, CUL1, CUL2, CUL5, NEDD8, CIDEB, DFFB, TOP2A,
Protein Size: 338
Molecular Weight 39 kDa
Gene ID 1677
NCBI Accession NM_004402, NP_004393
UniProt O76075
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB antibody (ABIN2784747) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 338 AA

Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB antibody (ABIN2784747) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DFFB antibody (ABIN2784747)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Neimanis, Albig, Doenecke, Kahle: "Sequence elements in both subunits of the DNA fragmentation factor are essential for its nuclear transport." in: The Journal of biological chemistry, Vol. 282, Issue 49, pp. 35821-30, 2007

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN2784747) back to top
Supplier Images
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (Middle Region) antibody (ABIN2784747) WB Suggested Anti-DFFB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...