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|Antigen||Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies|
|Reactivity||Human, Monkey Alternatives|
|Conjugate||This DIABLO antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-DIABLO AntibodyTarget Details DIABLO Application Details Handling Images
|Specificity||Recognizes Second Mitochondria-derived Activator of Caspase (SMAC/DIABLO).|
Species reactivity (expected):Monkey.
Species reactivity (tested):Human.
|Characteristics||Synonyms: Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, DirectIAP-binding protein with low pI|
|Purification||Protein G Chromatograghy.|
|Immunogen||Recombinant Human Smac/DIABLO protein.|
Target Details DIABLOProduct Details anti-DIABLO Antibody Application Details Handling Images back to top
|Alternative Name||DIABLO / SMAC (DIABLO Antibody Abstract)|
|Background||Second mitochondria-derived activator of caspase (SMAC) has been implicated in the activation of apoptosis in response to cell stress. SMAC promotes CASP9 activation by binding to IAPs and removing their inhibitory activity. The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Smac is an antagonist of the X-linked inhibitor of apoptosis (XIAP) - the most potent caspase inhibitor of all known inhibitor of apoptosis-family members. Smac promotes apoptosis by eliminating the inhibitory effect of inhibitor-of-apoptosis (IAPs) through physical interactions.Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Western Blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This DIABLO/SMAC antibody was validated for use in immunohistochemistry on a panel of21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
|Buffer||PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 50 % Glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top