anti-Human Endonuclease G antibody for Flow Cytometry

Recommended Endonuclease G Antibody (supplied by: Log in to see )

Antigen
Endonuclease G (ENDOG) Antibodies
  • CG8862
  • Dmel\\CG8862
  • Tb08.10J17.140
  • Tb08.10J17.90
  • TBC1D13
  • endog
  • ENDOG
  • wu:fb79c08
  • zgc:110020
  • Endonuclease G
  • endonuclease G
  • putative endonuclease G
  • endonuclease G, mitochondrial
  • endonuclease G L homeolog
  • EndoG
  • Tc00.1047053506867.10
  • Tb927.8.4040
  • Tb927.8.4090
  • LBRM_10_0750
  • LMJF_10_0610
  • NAEGRDRAFT_78507
  • ENDOG
  • endog
  • eNDOG
  • LOC100529218
  • Endog
  • endog.L
Epitope
AA 200-250
15
11
6
4
4
4
3
3
2
2
2
2
2
2
2
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
102
66
65
3
2
2
2
1
1
1
1
1
1
Host
Rabbit
80
27
Clonality
Polyclonal
Conjugate
This Endonuclease G antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
82
30
25
17
13
9
6
6
5
4
4
1
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Catalog No. ABIN1574189
$ 160.67
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.755681 ABIN3015250 FACS WB Rabbit Log in to see Polyclonal 0
12.362139 ABIN390490 FACS WB Rabbit Ig Fraction AA 188-217, C-Term Log in to see Polyclonal 0
12.362139 ABIN3002690 FACS WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN2720167 FACS IF IHC WB Mouse IgG1 Log in to see 5F1 0
1 ABIN1838793 FACS WB Rabbit AA 200-250 Log in to see Polyclonal 0
1 ABIN2450526 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 5F1 0
1 ABIN1841282 FACS WB Rabbit AA 188-217 Log in to see Polyclonal 0

General

Antigen Endonuclease G (ENDOG) Antibodies
Epitope AA 200-250
(15), (11), (6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity Rat (Rattus), Mouse (Murine), Human
(102), (66), (65), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(80), (27)
Clonality
Polyclonal
Conjugate This Endonuclease G antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(82), (30), (25), (17), (13), (9), (6), (6), (5), (4), (4), (1)
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Product Details anti-Endonuclease G Antibody

Target Details Endonuclease G Application Details Handling Images
Specificity EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.Positive Control: Ramos, mouse kidney
Cross-Reactivity (Details) EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.Positive Control: Ramos, mouse kidney
Purification Immunoaffinity chromatography
Immunogen KLH-coupled synthetic peptide within AA 200–250 of human EndoG
Isotype IgG
Plasmids, Primers & others

Target Details Endonuclease G

Product Details anti-Endonuclease G Antibody Application Details Handling Images back to top
Antigen
Alternative Name EndoG (ENDOG Antibody Abstract)
Background Endo G is an endonuclease that cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate the replication of mitochondrial DNA. EndoG is a mitochondrion-specific nuclease that cleaves chromatin DNA during apoptosis.Rabbit EndoG Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 200-50 of human EndoG (Swiss Prot: Q14249).
Pathways Apoptosis

Application Details

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Handling Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
Restrictions For Research Use only

Handling

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details Images back to top
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Images

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Endonuclease G (ENDOG) (AA 200-250) antibody (ABIN1574189) Western blot analysis of tissue lysates using EndoG Antibody (ABIN399086, 1 µg/mL) Th...
Flow Cytometry (FACS) image for anti-Endonuclease G (ENDOG) (AA 200-250) antibody (ABIN1574189) Flow cytometric analysis of Ramos cells using EndoG Antibody (ABIN399086, shaded hist...