anti-Rat (Rattus) Endonuclease G antibody for Western Blotting

Recommended Endonuclease G Antibody (supplied by: Log in to see )

Antigen
Endonuclease G (ENDOG) Antibodies
  • CG8862
  • Dmel\\CG8862
  • Tb08.10J17.140
  • Tb08.10J17.90
  • TBC1D13
  • endog
  • ENDOG
  • wu:fb79c08
  • zgc:110020
  • Endonuclease G
  • endonuclease G
  • endonuclease G, mitochondrial
  • endonuclease G, mitochondrial-like
  • EndoG
  • Tc00.1047053506867.10
  • Tb927.8.4040
  • Tb927.8.4090
  • LbrM10_V2.0750
  • LmjF10.0610
  • NAEGRDRAFT_78507
  • ENDOG
  • endog
  • eNDOG
  • LOC100410999
  • Endog
Epitope
Middle Region
15
11
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus), Zebrafish
97
67
64
4
1
1
1
1
1
1
1
1
Host
Rabbit
74
27
Clonality
Polyclonal
Conjugate
This Endonuclease G antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
78
29
20
19
13
8
6
5
4
2
2
1
Options
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Catalog No. ABIN2784751
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1438015 ABIN265775 IHC (p) IHC WB Rabbit Log in to see Polyclonal 2
3.1438015 ABIN4308033 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.1438015 ABIN680893 IF (p) IHC (p) WB Rabbit IgG AA 110-150 Log in to see Polyclonal
3.1438015 ABIN783844 WB Rabbit Log in to see Polyclonal
3.1438015 ABIN411479 WB Rabbit IgG Log in to see Polyclonal
3.1438015 ABIN2706097 WB Rabbit Center Log in to see Polyclonal
3.1438015 ABIN4903576 IHC WB Rabbit IgG Log in to see Polyclonal
3.1438015 ABIN633888 WB Rabbit Middle Region Log in to see Polyclonal
3.1438015 ABIN1574189 FACS WB Rabbit IgG AA 200-250 Log in to see Polyclonal
3.1438015 ABIN920903 IHC WB Rabbit Log in to see Polyclonal
3.1438015 ABIN1031370 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.1438015 ABIN1031747 ELISA WB Mouse Log in to see 7G1G10
3.1438015 ABIN1031746 ELISA WB Mouse Log in to see 7G1C10
3.1438015 ABIN1031749 ELISA WB Mouse Log in to see 7F2G10
3.1438015 ABIN1031748 ELISA WB Mouse Log in to see 7F2D7
3.1438015 ABIN190010 WB Rabbit AA 55-70 Log in to see Polyclonal
1 ABIN2473433 IHC (p) WB Rabbit IgG AA 55-70 Log in to see Polyclonal 2
1 ABIN5554776 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1453254 ELISA WB Rabbit IgG AA 61-110 Log in to see Polyclonal
1 ABIN469448 WB Rabbit C-Term Log in to see Polyclonal

General

Antigen Endonuclease G (ENDOG) Antibodies
Epitope Middle Region
(15), (11), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus), Zebrafish
(97), (67), (64), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(74), (27)
Clonality
Polyclonal
Conjugate This Endonuclease G antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(78), (29), (20), (19), (13), (8), (6), (5), (4), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-Endonuclease G Antibody

Target Details Endonuclease G Application Details Handling References for anti-Endonuclease G antibody (ABIN2784751) Images
Blocking Peptide Blocking peptide for this product available: ABIN976338
Sequence YVMPNAPVDE AIPLERFLVP IESIERASGL LFVPNILARA GSLKAITAGS
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against ENDOG. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ENDOG

Target Details Endonuclease G

Product Details anti-Endonuclease G Antibody Application Details Handling References for anti-Endonuclease G antibody (ABIN2784751) Images back to top
Antigen
Alternative Name ENDOG (ENDOG Antibody Abstract)
Background ENDOG cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities. ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Alias Symbols: FLJ27463
Protein Interaction Partner: UBC, BIRC2, DNAJA4, STUB1, HSPA4, AIFM1, FEN1,
Protein Size: 297
Molecular Weight 28 kDa
Gene ID 2021
NCBI Accession NM_004435, NP_004426
UniProt Q14249
Pathways Apoptosis

Application Details

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Handling References for anti-Endonuclease G antibody (ABIN2784751) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 297 AA

Restrictions For Research Use only

Handling

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details References for anti-Endonuclease G antibody (ABIN2784751) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Endonuclease G antibody (ABIN2784751)

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details Handling Images back to top
Product cited in:

Guha, Bandyopadhyaya, Polumuri, Chumsri, Gade, Kalvakolanu, Ahmed et al.: "Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell-like properties via a signaling cascade involving galectin-3, α9 nicotinic ..." in: Breast cancer research and treatment, Vol. 145, Issue 1, pp. 5-22, 2014

Background publications

Varecha, Amrichová, Zimmermann, Ulman, Lukásová, Kozubek: "Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells." in: Apoptosis : an international journal on programmed cell death, Vol. 12, Issue 7, pp. 1155-71, 2007

Images

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details Handling References for anti-Endonuclease G antibody (ABIN2784751) back to top
Supplier Images
Western Blotting (WB) image for anti-Endonuclease G (ENDOG) (Middle Region) antibody (ABIN2784751) WB Suggested Anti-ENDOG Antibody Titration: 0.2-1 ug/ml
ELISA Titer: