anti-Mouse (Murine) FASL antibody for Blocking Reagent

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • FASL
  • TNFSF6
  • fasl
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • FASLG
  • zgc:162027
  • Fas ligand
  • Fas ligand L homeolog
  • Fas ligand (TNF superfamily, member 6)
  • FASLG
  • faslg.L
  • Fasl
  • Faslg
  • faslg
Reactivity
Mouse (Murine), Rat (Rattus)
438
174
85
36
7
5
5
3
3
2
1
Host
Armenian Hamster
278
171
49
33
31
9
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
47
32
29
14
13
13
10
10
9
9
9
9
9
9
8
7
7
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS)
337
261
127
100
76
70
52
48
29
28
13
12
10
10
9
5
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2689023
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1176981 BR FACS Armenian Hamster IgG1, kappa Log in to see MFL3 17
1 ABIN1176980 BR FACS Armenian Hamster IgG3, kappa Log in to see MFL4 11

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(438), (174), (85), (36), (7), (5), (5), (3), (3), (2), (1)
Host Armenian Hamster
(278), (171), (49), (33), (31), (9), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(47), (32), (29), (14), (13), (13), (10), (10), (9), (9), (9), (9), (9), (9), (8), (7), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS)
(337), (261), (127), (100), (76), (70), (52), (48), (29), (28), (13), (12), (10), (10), (9), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL antibody (ABIN2689023) Images
Brand BD Pharmingen™
Characteristics The MFL4 antibody, generated against mouse CD178 (Fas Ligand, CD95 Ligand), cross-reacts with the rat Fas Ligand antigen. MFL4 mAb detects rat Fas Ligand expressed by transfected cells, but not the parental cell line. In addition, the epitope detected by MFL4 can be induced on activated rat splenic T lymphocytes. The MFL4 mAb has been reported to inhibit killing of mouse lymphoma line A20.2J by rat FasL-transfected cells. The tissue distribution of mouse Fas Ligand has been well characterized. It is expressed at the mRNA and protein levels in a variety of tissues and cell types, including spleen, bone marrow, testis, uterus, eye, and activated lymphocytes. The expression of Fas Ligand in the rat has been detected in liver Kupffer cells, T cells in the central nervous system, spleen, and corpus luteum. MFL4 antibody reacts with the mouse Fas Ligand protein in a pattern similar to that of MFL3 antibody (Cat. No. 555291). Fas Ligand is a type-II transmembrane protein, a member of the TNF/NGF superfamily and a ligand for Fas (CD95). Fas Ligand and its counter-receptor CD95 are believed to participate in T-cell development, the regulation of immune responses, and cell-mediated cytotoxic mechanisms. It has been reported that human Fas Ligand is released from the surface of activated lymphocytes by metalloproteinase. Expression of Fas Ligand on activated T lymphocytes. T lymphocytes from LOU spleen (rat T Cell Enrichment Column, R&D Systems, Minneapolis, MN) were cultured for 7 hours in the presence of plate-bound R73 and JJ316 antibodies (anti-αβ TCR, Cat. No. 554910, and anti-CD28, Cat. No. 554992, respectively, top panels) or on uncoated plates (bottom panels). They were simultaneously stained with FITC-conjugated OX-35 (anti-rat CD4, Cat. No. 554837, all panels) and purified MFL4 (right panels) or purified A19-4 (isotype control, Cat. No. 553977, left panels) antibodies, followed by biotinylated goat anti-Armenian hamster IgG (Jackson Immunoresearch, West Grove, PA), then Streptavidin-PE (Cat. No. 554061, all panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system. 555022 Rev. 6 Page 1 of 2

BD Pharmingen™ Purified Hamster Anti-Mouse and Rat CD178 - Purified - Clone MFL4 - Isotype Armenian Hamster IgG3, κ - Reactivity Rat, Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse FasL transfected cells
Clone MFL4
Isotype IgG3, kappa

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL antibody (ABIN2689023) Images back to top
Antigen
Alternative Name CD178 (FASL Antibody Abstract)
Background Synonyms: Fas Ligand, CD95 Ligand
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL antibody (ABIN2689023) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL antibody (ABIN2689023) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-FASL antibody (ABIN2689023)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Product cited in:

Trinité, Voisine, Yagita, Josien: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, 2000

Moalem, Monsonego, Shani, Cohen, Schwartz: "Differential T cell response in central and peripheral nerve injury: connection with immune privilege." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 13, Issue 10, pp. 1207-17, 1999

Müschen, Warskulat, Douillard, Gilbert, Häussinger: "Regulation of CD95 (APO-1/Fas) receptor and ligand expression by lipopolysaccharide and dexamethasone in parenchymal and nonparenchymal rat liver cells." in: Hepatology (Baltimore, Md.), Vol. 27, Issue 1, pp. 200-8, 1998

White, McCombe, Pender: "The roles of Fas, Fas ligand and Bcl-2 in T cell apoptosis in the central nervous system in experimental autoimmune encephalomyelitis." in: Journal of neuroimmunology, Vol. 82, Issue 1, pp. 47-55, 1998

Kayagaki, Yamaguchi, Nagao, Matsuo, Maeda, Okumura, Yagita: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997

Griffith, Brunner, Fletcher, Green, Ferguson: "Fas ligand-induced apoptosis as a mechanism of immune privilege." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1189-92, 1996

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996

Brunner, Mogil, LaFace, Yoo, Mahboubi, Echeverri, Martin, Force, Lynch, Ware: "Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas." in: Nature, Vol. 373, Issue 6513, pp. 441-4, 1995

Suda, Okazaki, Naito, Yokota, Arai, Ozaki, Nakao, Nagata: "Expression of the Fas ligand in cells of T cell lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3806-13, 1995

Vignaux, Vivier, Malissen, Depraetere, Nagata, Golstein: "TCR/CD3 coupling to Fas-based cytotoxicity." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 781-6, 1995

Smith, Farrah, Goodwin: "The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death." in: Cell, Vol. 76, Issue 6, pp. 959-62, 1994