anti-Cat (Feline) KRT18 antibody for Immunohistochemistry (Frozen Sections)

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human, Dog (Canine), Cat (Feline)
1139
248
231
135
97
89
62
59
44
43
39
34
29
29
29
27
25
24
6
4
3
2
2
1
1
1
1
Host
Mouse
883
284
14
13
4
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
61
51
29
21
20
20
18
17
14
14
14
14
14
14
14
13
13
11
11
9
8
8
8
8
8
8
8
8
8
8
8
8
8
4
3
3
3
3
3
3
3
3
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
715
563
525
461
443
305
221
170
161
120
45
41
18
14
11
11
3
3
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN335341
$ 376.36
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106920 IHC (fro) FACS IF IHC (p) WB Mouse IgG1 Log in to see DE-K18 4
1 ABIN200745 FACS ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 0

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human, Dog (Canine), Cat (Feline)
(1139), (248), (231), (135), (97), (89), (62), (59), (44), (43), (39), (34), (29), (29), (29), (27), (25), (24), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(883), (284), (14), (13), (4), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(61), (51), (29), (21), (20), (20), (18), (17), (14), (14), (14), (14), (14), (14), (14), (13), (13), (11), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(715), (563), (525), (461), (443), (305), (221), (170), (161), (120), (45), (41), (18), (14), (11), (11), (3), (3), (2), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN335341) Images
Specificity Human, canine and feline.
Purification Purified
Immunogen DE-K18 is a mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 mouse immunized with a cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431.
Clone DE-K18
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN335341) Images back to top
Antigen
Alternative Name Cytokeratin 18 / Keratin K18 (KRT18 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN335341) Images back to top
Application Notes DE-K18 reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. DE-K18 is useful for immunocytochemistry, immunoblotting, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. For staining on paraffin- embedded tissues pretreatment with 0,1% pepsin in 0.1 N HCl 30 min at room temperature is required. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN335341) Images back to top
Storage 4 °C

References for anti-KRT18 antibody (ABIN335341)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Bonfrer, Groeneveld, Korse, van Dalen, Oomen, Ivanyi: "Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18." in: Tumour biology, Vol. 15, Issue 4, pp. 210-22, 1994

van Bommel, Kenemans, Helmerhorst, Gallee, Ivanyi: "Expression of cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix." in: Cancer, Vol. 74, Issue 8, pp. 2314-20, 1994

Vos, van den Ingh, de Neijs, van Mil, Ivanyi, Ramaekers: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." in: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, 1993

Vos, van den Ingh, Ramaekers, Molenbeek, de Neijs, van Mil, Ivanyi: "The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region." in: Veterinary pathology, Vol. 30, Issue 4, pp. 352-61, 1993

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, 1992