anti-Dog (Canine) KRT18 antibody for Western Blotting

Recommended KRT18 Antibody

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human, Mouse (Murine), Rat (Rattus), Hamster, Rabbit, Chicken, Dog (Canine), Pig (Porcine), Zebrafish (Danio rerio)
899
216
210
116
86
60
59
45
42
36
32
27
27
27
25
14
10
8
6
4
3
2
1
1
1
1
Host
Mouse
590
336
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
56
41
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
568
379
347
346
260
200
171
97
82
79
42
40
18
14
11
1
1
1
Options

Available images

Catalog No. ABIN335330
$ 383.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.890326 ABIN2778669 IHC WB Rabbit N-Term Polyclonal 1
19 ABIN1491639 IHC IHC (p) WB Mouse IgG2b AA 69-372 5E8 0
18.051027 ABIN335341 ICC FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 DE-K18 5
16.890326 ABIN2778670 IHC WB Rabbit C-Term Polyclonal 1
16 ABIN959489 FACS ICC IHC IHC (fro) WB Mouse IgG1 RGE53 0
14.5 ABIN111183 IHC (fro) IHC (p) WB Mouse IgG1 Ks18-04 14
13.551027 ABIN933344 IF IHC WB Mouse IgG1 RGE53 0
13.551027 ABIN5617705 FACS IHC IHC (fro) WB Mouse IgG1 RGE53 0
10 ABIN2719066 IHC WB Mouse IgG2b AA 69-372 5E8 0
9.446655 ABIN4302603 CyTOF FACS ICC IF IHC IHC (fro) WB Mouse IgG1 RGE53 1
7 ABIN203132 IHC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
7 ABIN3026958 FACS IF IHC (p) WB Mouse IgG1, kappa DE-K18 0
7 ABIN3026966 FACS IF IHC (p) WB Mouse IgG1, kappa C-04 or Ks18-04 0
7 ABIN3026960 FACS IF IHC (p) WB Mouse IgG1, kappa DE-K18 0
7 ABIN3026968 FACS IF IHC (p) WB Mouse IgG1, kappa C-04 or Ks18-04 0
4 ABIN959458 FACS ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 DE-K18 0
4 ABIN1724420 IHC (f) ICC FACS IHC (p) WB Mouse IgG1 DE-K18 0
1 ABIN1106918 IHC (fro) FACS IF WB Mouse IgG1 RGE53 9
1 ABIN1106920 IHC (fro) FACS IF IHC (p) WB Mouse IgG1 DE-K18 4
1 ABIN452377 IHC (fro) IHC (p) WB Mouse IgG1 multi Epitope Ks18-04-18-27-18-5-9B1-18-174 0

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus), Hamster, Rabbit, Chicken, Dog (Canine), Pig (Porcine), Zebrafish (Danio rerio)
(899), (216), (210), (116), (86), (60), (59), (45), (42), (36), (32), (27), (27), (27), (25), (14), (10), (8), (6), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(590), (336), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(56), (41), (18), (11), (11), (11), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3), (3), (3), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
(568), (379), (347), (346), (260), (200), (171), (97), (82), (79), (42), (40), (18), (14), (11), (1), (1), (1)
Pubmed 8 references available
Supplier

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN335330) Images
Specificity Human, mouse, rat, hamster, rabbit, chicken, canine and swine.
Purification Purified
Immunogen RGE53 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal preparation of cells.
Clone RGE53
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN335330) Images back to top
Antigen
Alternative Name Cytokeratin 18 / Keratin K18 (KRT18 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN335330) Images back to top
Application Notes RGE53 reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. RGE53 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN335330) Images back to top
Storage 4 °C

References for anti-KRT18 antibody (ABIN335330)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

van Leenders, Dijkman, Hulsbergen-van de Kaa, Ruiter, Schalken: "Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 8, pp. 1251-8, 2000

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Puts, Moesker, Kenemans, Vooijs, Ramaekers: "Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 4, Issue 4, pp. 300-13, 1986

Ramaekers, Puts, Moesker, Kant, Huysmans, Haag, Jap, Herman, Vooijs: "Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview." in: The Histochemical journal, Vol. 15, Issue 7, pp. 691-713, 1983

Ramaekers, Huysmans, Moesker, Kant, Jap, Herman, Vooijs: "Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 49, Issue 3, pp. 353-61, 1983

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN335330) back to top
Supplier Images
 image for anti-Keratin 18 (KRT18) antibody (ABIN335330) anti-Keratin 18 (KRT18) antibody
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Keratin 18 (KRT18) antibody (ABIN335330) Immunohistochemistry on frozen section of human kidney epithelium