anti-Human KRT18 antibody for Enzyme Immunoassay

Recommended KRT18 Antibody

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human
899
217
211
117
86
61
59
46
42
36
32
27
27
27
25
15
11
9
6
4
3
2
1
1
1
1
Host
Mouse
590
336
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
56
41
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
568
378
348
347
259
200
171
98
81
80
42
40
18
14
10
1
1
1
Options
Catalog No. ABIN120626
$ 258.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10 ABIN118968 EIA IF IHC (p) IP WB Mouse IgG1 DC-10 7
10 ABIN567623 EIA IF IHC (p) WB Mouse IgG2b AT2G3 0
7 ABIN614437 EIA IHC (p) WB Mouse IgG2b AA 391-483 4D11E4 0
7 ABIN265498 EIA IHC (p) IP WB Rabbit Polyclonal 0
4 ABIN452897 EIA WB Rabbit C-Term Polyclonal 2
1 ABIN125989 EIA IHC (fro) WB Mouse IgG1 Ks18-27 3
1 ABIN118967 EIA IF IHC (p) WB Biotin Mouse IgG1 DC-10 3
1 ABIN187683 EIA IF IHC (p) WB Mouse IgG1 DA-7 0

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human
(899), (217), (211), (117), (86), (61), (59), (46), (42), (36), (32), (27), (27), (27), (25), (15), (11), (9), (6), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(590), (336), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(56), (41), (18), (11), (11), (11), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3), (3), (3), (2), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(568), (378), (348), (347), (259), (200), (171), (98), (81), (80), (42), (40), (18), (14), (10), (1), (1), (1)
Pubmed 7 references available
Supplier

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN120626) Images
Specificity The antibody DA-7 reacts with Cytokeratin 18 (45 kDa).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Immunogen Human breast carcinoma cell line PMC-42
Clone DA-7
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN120626) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN120626) Images back to top
Application Notes Immunoprecipitation. Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail), Incubate 60 min on ice, Centrifuge to remove cell debris, Mix lysate with non-reducing SDS-PAGE sample buffer. Positive control: HeLa human cervix carcinoma cell line, A-431 human epidermoid carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry on paraffin sections: 1:10 - 1:100. Positive tissue: epithelial tissue (skin). Immunocytochemistry. Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line. ELISA: The antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04. Positive control: MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN120626) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months

References for anti-KRT18 antibody (ABIN120626)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Kaulfürst-Soboll, Mertens, Brehler, von Schaewen: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, 2011

Cebecauer, Cerný, Horejsí: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, 1998

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990

Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, 1990

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, 1987

Horejsí, Hilgert, Kristofová, Bazil, Bukovský, Kulhánková et al.: "Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte ..." in: Folia biologica, Vol. 32, Issue 1, pp. 12-25, 1986