anti-Human KRT18 antibody for Flow Cytometry

Recommended KRT18 Antibody

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human
913
217
215
117
86
61
59
46
42
36
32
27
27
27
26
15
11
9
6
4
3
2
1
1
1
1
Host
Mouse
598
344
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is conjugated to FITC
56
41
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
584
381
357
349
264
205
171
102
86
80
45
40
18
14
11
3
1
1
1
Options

Available images

Catalog No. ABIN302009
$ 454.57
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
28.840088 ABIN676973 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 70-110 Polyclonal 2
28.593636 ABIN335330 ICC FACS IHC (fro) IHC WB Mouse IgG1 RGE53 8
25.593636 ABIN335331 ICC FACS IHC (fro) IHC WB Mouse IgG1 RCK106 10
24.340088 ABIN391561 FACS IF IHC (p) WB Rabbit Ig Fraction AA 401-430, C-Term Polyclonal 0
24.340088 ABIN3031537 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 401-430 Polyclonal 0
24.093636 ABIN94282 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 DC-10 4
24.093636 ABIN94283 ELISA FACS ICC IHC (p) IP WB Biotin Mouse IgG1 DC-10 3
19 ABIN6179385 FACS IF IHC (f) PerCP Mouse IgG1 KRT18-834 0
19 ABIN6179378 FACS IF IHC (f) APC Mouse IgG1 KRT18-834 0
19 ABIN6179386 FACS IF IHC (f) PE Mouse IgG1 KRT18-834 0
19 ABIN6179389 FACS IF IHC (f) Mouse IgG1 KRT18-834 0
19 ABIN3026947 FACS IF IHC (p) Mouse IgG1 KRT18-834 0
19 ABIN3026949 FACS IF IHC (p) Mouse IgG1 KRT18-834 0
18.093636 ABIN335341 ICC FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 DE-K18 5
16 ABIN6179423 FACS IF IHC (f) PerCP Mouse IgG1 KRT18-835 0
16 ABIN6179424 FACS IF IHC (f) PE Mouse IgG1 KRT18-835 0
16 ABIN6179416 FACS IF IHC (f) APC Mouse IgG1 KRT18-835 0
16 ABIN6179427 FACS IF IHC (f) Mouse IgG1 KRT18-835 0
16 ABIN959489 FACS ICC IHC IHC (fro) WB Mouse IgG1 RGE53 0
16 ABIN3026950 FACS IF IHC (p) Mouse IgG1 KRT18-835 0

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human
(913), (217), (215), (117), (86), (61), (59), (46), (42), (36), (32), (27), (27), (27), (26), (15), (11), (9), (6), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(598), (344), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is conjugated to FITC
(56), (41), (18), (11), (11), (11), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3), (3), (3), (2), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(584), (381), (357), (349), (264), (205), (171), (102), (86), (80), (45), (40), (18), (14), (11), (3), (1), (1), (1)
Pubmed 3 references available
Supplier

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN302009) Images
Specificity The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
No Cross-Reactivity Pig (Porcine), Mouse (Murine), Rat (Rattus), Hamster, Cow (Bovine), Dog (Canine), Sheep (Ovine)
Cross-Reactivity (Details) Human
Immunogen Human breast carcinoma cell line PMC-42.
Clone DC-10
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN302009) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN302009) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis and Immunofluorescence.Suggested working dilution is 5-10 μg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN302009) Images back to top
Concentration 0.1 mg/mL
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-KRT18 antibody (ABIN302009)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN302009) back to top
Supplier Images
Western Blotting (WB) image for anti-Keratin 18 (KRT18) antibody (FITC) (ABIN302009) Detectionof cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10 .
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) antibody (FITC) (ABIN302009) Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokerat...
Flow Cytometry (FACS) image for anti-Keratin 18 (KRT18) antibody (FITC) (ABIN302009) Intracellular staining of HeLa cells with anti-cytokeratin 18 (DC-10) FITC.