anti-Mammalian KRT18 antibody for Cytometry by Time of Flight

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human, Mammalian, Mouse (Murine)
1141
247
231
136
96
90
62
59
44
42
39
34
29
29
29
27
25
24
6
4
3
2
2
1
1
1
1
Host
Mouse
885
284
14
13
4
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
61
51
29
21
20
20
18
17
14
14
14
14
14
14
14
13
13
11
11
9
8
8
8
8
8
8
8
8
8
8
8
8
8
4
3
3
3
3
3
3
3
3
2
1
Application
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
717
565
524
461
445
304
223
170
164
119
45
41
18
14
11
10
3
3
2
1
1
1
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Catalog No. ABIN268940
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General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human, Mammalian, Mouse (Murine)
(1141), (247), (231), (136), (96), (90), (62), (59), (44), (42), (39), (34), (29), (29), (29), (27), (25), (24), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(885), (284), (14), (13), (4), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(61), (51), (29), (21), (20), (20), (18), (17), (14), (14), (14), (14), (14), (14), (14), (13), (13), (11), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(717), (565), (524), (461), (445), (304), (223), (170), (164), (119), (45), (41), (18), (14), (11), (10), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images
Specificity The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Purification Protein A purified
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431.
Clone C-04
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Gene Symbol: KRT18
Gene ID 3875
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN268940) Images back to top
Application Notes Western Blot 1 - 2 μg/mL, Flow Cytometry 1:10-1:1000, ELISA 1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50, Immunohistochemistry-Paraffin 10 μg/mL, CyTOF-readyPredicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10 % separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN268940) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-KRT18 antibody (ABIN268940)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Kaenel, Antonijevic, Richter, Küchler, Sutter, Wotzkow, Strange, Andres: "Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway." in: International journal of oncology, Vol. 40, Issue 2, pp. 357-69, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [ABIN268940] - Human ki...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [ABIN268940] - Human Colon
Western Blotting (WB) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Western Blot: Cytokeratin 18 Antibody (C-04) - Analysis of cytokeratin 18 in HeLa ce...
Flow Cytometry (FACS) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Flow Cytometry: Cytokeratin 18 Antibody (C-04) - Intracellular flow cytometry analys...