anti-Mammalian KRT18 antibody for Enzyme Immunoassay

Recommended KRT18 Antibody

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Epitope
N-Term
35
34
33
17
16
15
11
10
8
8
8
8
7
5
5
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mammalian
914
217
215
117
86
61
59
46
42
36
32
27
27
27
25
15
11
9
6
4
3
2
1
1
1
1
Host
Mouse
598
344
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
56
42
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
584
381
357
350
265
206
171
103
87
79
45
40
18
14
10
3
1
1
1
Options
Catalog No. ABIN371615
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN120625 EIA FACS IF IHC (p) IP WB Mouse IgG1 C-04 6

General

Antigen Keratin 18 (KRT18) Antibodies
Epitope N-Term
(35), (34), (33), (17), (16), (15), (11), (10), (8), (8), (8), (8), (7), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mammalian
(914), (217), (215), (117), (86), (61), (59), (46), (42), (36), (32), (27), (27), (27), (25), (15), (11), (9), (6), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(598), (344), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(56), (42), (18), (11), (11), (11), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3), (3), (3), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(584), (381), (357), (350), (265), (206), (171), (103), (87), (79), (45), (40), (18), (14), (10), (3), (1), (1), (1)
Pubmed 7 references available
Supplier

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN371615) Images
Specificity This antibody reacts specifically specifically with Keratin 18 and recombinant N-terminal Keratin 18.
Characteristics Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18, Cell proliferation-inducinggene 46 protein, Keratin type I cytoskeletal 18
Purification Purified
Immunogen Recombinant N-terminal fragment of keratin 18.
Clone SB38b
Isotype IgG2b
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN371615) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30 % sequence homology. (Ref.1-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein of approximately 48 kDa. It exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia, and is often co-expressed with cytokeratin 8. (Ref.6-8) Tissues from the gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN371615) Images back to top
Application Notes ELISA: 1/5,000-1/20,000. Western Blot: 1/2,000-1/4,000. Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections): 10 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN371615) Images back to top
Concentration 0.1 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.0 without preservatives or amine-containing buffer salts.
Preservative Without preservative
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-KRT18 antibody (ABIN371615)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Background publications

Ku, Darling, Krams, Esquivel, Keeffe, Sibley, Lee, Wright, Omary: "Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 10, pp. 6063-8, 2003

Kulesh, Oshima: "Complete structure of the gene for human keratin 18." in: Genomics, Vol. 4, Issue 3, pp. 339-47, 1989

Steinert, Roop: "Molecular and cellular biology of intermediate filaments." in: Annual review of biochemistry, Vol. 57, pp. 593-625, 1988

Schiller, Franke, Geiger: "A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells." in: The EMBO journal, Vol. 1, Issue 6, pp. 761-9, 1984

Hanukoglu, Fuchs: "The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins." in: Cell, Vol. 33, Issue 3, pp. 915-24, 1983

Tseng, Jarvinen, Nelson, Huang, Woodcock-Mitchell, Sun: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983

Fuchs, Coppock, Green, Cleveland: "Two distinct classes of keratin genes and their evolutionary significance." in: Cell, Vol. 27, Issue 1 Pt 2, pp. 75-84, 1982