anti-Mammalian KRT18 antibody for Immunofluorescence
Recommended KRT18 Antibody (supplied by: Log in to see )
- Antigen
-
Keratin 18 (KRT18) Antibodies
show synonyms for this antigen- CK18
- K18
- Krt1-18
- CYK18
- DreK18
- cb83
- dapk1
- sb:cb83
- wu:fa13f02
- wu:fb36b04
- krt18
- MGC64569
- MGC75922
- KRT18
- keratin 18
- keratin 18, type I L homeolog
- keratin 18, type I
- keratin, type I cytoskeletal 18
- K18, simple type I keratin
- Keratin, type I cytoskeletal 18
- Krt18
- KRT18
- krt18
- krt18.L
- LOC700569
- LOC100136778
- k1c18
- LOC100008885
- Reactivity
-
Human, Mammalian, Mouse (Murine)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Conjugate
-
This KRT18 antibody is un-conjugated
Alternatives - Application
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Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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Available images
Catalog No. ABIN268940
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN120625 | EIA FACS IF IHC (p) IP WB | Mouse | IgG1 | Log in to see | C-04 | 6 | |||
| 1 | ABIN470782 | IF IHC IHC (fro) WB | Mouse | IgG1 | Log in to see | 0 | ||||
| 1 | ABIN181159 | IHC (fro) IF WB | Mouse | IgG1 | Log in to see | RGE53 | 0 | |||
| 1 | ABIN470783 | IF IHC IHC (fro) WB | Mouse | IgG1 | Log in to see | 0 | ||||
| -12.939373 | ABIN5950069 | FACS ICC IF IHC IHC (p) | Janelia Fluor® 646 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN5950068 | FACS ICC IF IHC IHC (p) | Janelia Fluor® 549 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302594 | ELISA FACS ICC IF IHC IHC (p) IP WB | FITC | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302608 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 405 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302620 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 755 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302642 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 405 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302645 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 700 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302644 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 647 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302614 | ELISA FACS ICC IF IHC IHC (p) IP WB | PerCP | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302624 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 488 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302605 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 350 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302623 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 680 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
| -12.939373 | ABIN4302643 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 488 | Mouse | IgG1 | Log in to see | C-04 | 0 |
General |
|
|---|---|
| Antigen | Keratin 18 (KRT18) Antibodies |
| Reactivity | Human, Mammalian, Mouse (Murine) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This KRT18 antibody is un-conjugated Alternatives |
| Application |
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
|
|
1 reference available |
| Supplier | Log in to see |
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images |
|
| Specificity | The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues. |
| Purification | Protein A purified |
| Immunogen | Cytoskeleton preparation of epidermal carcinoma cell line A431. |
| Clone | C-04 |
| Isotype | IgG1 |
| Plasmids, Primers & others | |
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images back to top |
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| Antigen | |
| Alternative Name | Cytokeratin 18 (KRT18 Antibody Abstract) |
| Background | Gene Symbol: KRT18 |
| Gene ID | 3875 |
| Pathways | Apoptosis, Caspase Cascade in Apoptosis |
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN268940) Images back to top |
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| Application Notes | Western Blot 1 - 2 μg/mL, Flow Cytometry 1:10-1:1000, ELISA 1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50, Immunohistochemistry-Paraffin 10 μg/mL, CyTOF-readyPredicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10 % separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. |
| Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN268940) Images back to top |
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| Format | Liquid |
| Concentration | 1 mg/mL |
| Buffer |
PBS ( pH 7.4) Buffer contains: 15 mM Sodium Azide |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice | Avoid freeze-thaw cycles |
| Storage | 4 °C |
| Storage Comment | Store at 4°C. Do not freeze. |
References for anti-KRT18 antibody (ABIN268940)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top |
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| Product cited in: |
Kaenel, Antonijevic, Richter, Küchler, Sutter, Wotzkow, Strange, Andres: "Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway." in: International journal of oncology, Vol. 40, Issue 2, pp. 357-69, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections) |
ImagesProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) back to top |
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| Supplier Images |
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [ABIN268940] - Human ki...
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [ABIN268940] - Human Colon
Western Blot: Cytokeratin 18 Antibody (C-04) - Analysis of cytokeratin 18 in HeLa ce...
Flow Cytometry: Cytokeratin 18 Antibody (C-04) - Intracellular flow cytometry analys...
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