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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN120625 | EIA FACS IF IHC (p) IP WB | Mouse | IgG1 | Log in to see | C-04 | 6 | |||
1 | ABIN470782 | IHC (fro) IF WB | Mouse | IgG1 | Log in to see | 0 | ||||
1 | ABIN4302594 | ELISA FACS ICC IF IHC IHC (p) IP WB | FITC | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302605 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 350 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302608 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 405 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302624 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 488 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302642 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 405 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302614 | ELISA FACS ICC IF IHC IHC (p) IP WB | PerCP | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302623 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 680 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302620 | ELISA FACS ICC IF IHC IHC (p) WB | DyLight 755 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302645 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 700 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302643 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 488 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN4302644 | ELISA FACS ICC IF IHC IHC (p) WB | Alexa Fluor 647 | Mouse | IgG1 | Log in to see | C-04 | 0 | ||
1 | ABIN181159 | IHC (fro) IF WB | Mouse | IgG1 | Log in to see | RGE53 | 0 | |||
1 | ABIN470783 | IHC (fro) IF WB | Mouse | IgG1 | Log in to see | 0 |
General |
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Antigen | Keratin 18 (KRT18) Antibodies |
Reactivity | Human, Mammalian, Mouse (Murine) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This KRT18 antibody is un-conjugated Alternatives |
Application |
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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1 reference available |
Supplier | Log in to see |
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images |
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Specificity | The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues. |
Purification | Protein A purified |
Immunogen | Cytoskeleton preparation of epidermal carcinoma cell line A431. |
Clone | C-04 |
Isotype | IgG1 |
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images back to top |
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Antigen | |
Alternative Name | Cytokeratin 18 (KRT18 Antibody Abstract) |
Background | Gene Symbol: KRT18 |
Gene ID | 3875 |
Pathways | Apoptosis, Caspase Cascade in Apoptosis |
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN268940) Images back to top |
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Application Notes | Western Blot 1 - 2 μg/mL, Flow Cytometry 1:10-1:1000, ELISA 1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50, Immunohistochemistry-Paraffin 10 μg/mL, CyTOF-readyPredicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10 % separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN268940) Images back to top |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer |
PBS ( pH 7.4) Buffer contains: 15 mM Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C |
Storage Comment | Store at 4°C. Do not freeze. |
References for anti-KRT18 antibody (ABIN268940)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top |
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Product cited in: |
Kaenel, Antonijevic, Richter, Küchler, Sutter, Wotzkow, Strange, Andres: "Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway." in: International journal of oncology, Vol. 40, Issue 2, pp. 357-69, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections) |
ImagesProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) back to top |
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Supplier Images |
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