anti-Sheep (Ovine) KRT18 antibody for Western Blotting

Recommended KRT18 Antibody

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine), Trout, Zebrafish (Danio rerio)
927
224
216
126
96
66
64
51
47
38
36
33
33
33
25
15
11
8
5
4
3
2
1
1
1
1
Host
Mouse
614
340
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
56
41
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
595
404
378
375
258
200
171
98
82
78
42
40
30
18
14
11
2
1
1
1
Options
Catalog No. ABIN111183
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13.447434 ABIN6935331 FACS IF IHC StM WB Mouse IgG1, kappa rKRT18-1190 0
13.447434 ABIN6935343 FACS IF IHC StM WB Rabbit IgG KRT18-2819R 0
13.447434 ABIN6935339 FACS IF IHC StM WB Mouse IgG1, kappa C-04-same as Ks18-04 0
13.447434 ABIN6937676 FACS IF IHC StM WB Mouse IgG1, kappa rKRT18-1190 0
13.447434 ABIN6937689 FACS IF IHC StM WB Rabbit IgG KRT18-2819R 0
13.447434 ABIN6937685 FACS IF IHC StM WB Mouse IgG1, kappa C-04-same as Ks18-04 0
7 ABIN3026966 FACS IF IHC (p) WB Mouse IgG1, kappa C-04 or Ks18-04 0
7 ABIN3026968 FACS IF IHC (p) WB Mouse IgG1, kappa C-04 or Ks18-04 0
1 ABIN452377 IHC (fro) IHC (p) WB Mouse IgG1 multi Epitope Ks18-04-18-27-18-5-9B1-18-174 0
1 ABIN1824104 IHC WB Mouse IgG1 Ks18-04 0
1 ABIN6252486 FACS IF IHC (p) WB Mouse IgG1, kappa C-04 or Ks18-04 0
1 ABIN215746 IHC IHC (fro) IHC (p) WB Mouse IgG1 0
1 ABIN227747 ELISA IHC IHC (fro) IHC (p) WB Biotin Mouse IgG1 0
1 ABIN6295940 FACS IF IHC WB Mouse 0
1 ABIN470785 IHC IHC (fro) IHC (p) WB Mouse IgG1 0
1 ABIN5954699 ICC IF IHC WB Mouse IgG1 Ks18-04 0
1 ABIN5954700 ICC IF IHC WB Mouse IgG1 Ks18-04 0
1 ABIN5954697 ELISA ICC IF IHC WB Biotin Mouse IgG1 Ks18-04 0

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine), Trout, Zebrafish (Danio rerio)
(927), (224), (216), (126), (96), (66), (64), (51), (47), (38), (36), (33), (33), (33), (25), (15), (11), (8), (5), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(614), (340), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(56), (41), (18), (11), (11), (11), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3), (3), (3), (2), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(595), (404), (378), (375), (258), (200), (171), (98), (82), (78), (42), (40), (30), (18), (14), (11), (2), (1), (1), (1)
Pubmed 14 references available
Supplier

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN111183) Images
Specificity Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish
Purification Protein A chromatography
Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Clone Ks18-04
Isotype IgG1
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN111183) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
NCBI Accession NP_000215
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN111183) Images back to top
Application Notes Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN111183) Images back to top
Reconstitution Restore in 1.0 mL distilled water.
Buffer PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing). Dilute only prior to immediate use.
Storage -20 °C
Storage Comment Store the antiserum at -20 °C (aliquot contents to

References for anti-KRT18 antibody (ABIN111183)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Zatloukal, Kufferath, Thueringer, Landegren, Zatloukal, Haybaeck: "Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies." in: PLoS ONE, Vol. 9, Issue 5, pp. e96690, 2014

Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." in: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, 2012

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, 2012

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, 2005

Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, 2003

Schaffeld, Knappe, Hunzinger, Markl: "cDNA sequences of the authentic keratins 8 and 18 in zebrafish." in: Differentiation; research in biological diversity, Vol. 71, Issue 1, pp. 73-82, 2003

Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, 2002

Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, 2001

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, 1993

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983

Background publications

Bredt: "Nitric oxide signaling specificity--the heart of the problem." in: Journal of cell science, Vol. 116, Issue Pt 1, pp. 9-15, 2002