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|Antigen||Lamin A/C (LMNA) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This Lamin A/C antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Lamin A/C AntibodyTarget Details Lamin A/C Application Details Handling References for anti-Lamin A/C antibody (ABIN112346) Images
|Specificity||131C3 reacts with an epitope located between residues 319-566 in Lamin A and C. Immunoblotting of 131C3 recognizes Nuclear Lamins A and C in Human Fibroblasts.|
|Immunogen||Purified Rat liver Lamins.|
Target Details Lamin A/CProduct Details anti-Lamin A/C Antibody Application Details Handling References for anti-Lamin A/C antibody (ABIN112346) Images back to top
|Alternative Name||Lamin-A/C (LMNA) (LMNA Antibody Abstract)|
|Background||Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Handling References for anti-Lamin A/C antibody (ABIN112346) Images back to top
Immunoblotting: 1/100-1/1000. Flow Cytometry: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Frozen Sections: 1/100-1/200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details References for anti-Lamin A/C antibody (ABIN112346) Images back to top
|Buffer||PBS, 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Lamin A/C antibody (ABIN112346)Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling Images back to top
Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999
Neri, Raymond, Giordano, Borgatti, Marchisio, Capitani, Martelli: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999
Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997