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|Antigen||Lamin A/C (LMNA) Antibodies|
|Epitope||AA 455-469, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Lamin A/C antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-Lamin A/C AntibodyTarget Details Lamin A/C Application Details Handling References for anti-Lamin A/C antibody (ABIN3043695) Images
|Purpose||Rabbit IgG polyclonal antibody for Prelamin-A/C(LMNA) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Prelamin-A/C(LMNA) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: lamin A/C
Protein Name: Prelamin-A/C
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C(455-469aa RNKSNEDQSMGNWQI), identical to the related rat and mouse sequences.|
|Plasmids, Primers & others|
Target Details Lamin A/CProduct Details anti-Lamin A/C Antibody Application Details Handling References for anti-Lamin A/C antibody (ABIN3043695) Images back to top
|Alternative Name||LMNA (LMNA Antibody Abstract)|
Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C(LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.
Synonyms: 70 kDa Lamin antibody|Cardiomyopathy dilated 1A(autosomal dominant) antibody|CDCD1 antibody|CDDC antibody|Charcot Marie Tooth Disease Axonal Type 2B1 antibody|CMD1A antibody|CMT2B1 antibody|EMD2 antibody|FPL antibody|FPLD antibody|FPLD2 antibody|HGPS antibody|IDC antibody|Lamin A/C antibody|Lamin A/C Isoform 1 Precursor antibody|Lamin A/C Isoform 2 antibody|Lamin A/C Isoform 3 antibody|Lamin A/C like 1 antibody|Lamin-A/C antibody|LDP1 antibody|LFP antibody|LGMD1B antibody|Limb girdle muscular dystrophy 1B(autosomal dominant) antibody|LMN 1 antibody|LMN A antibody|LMN C antibody|LMN1 antibody|LMNA antibody|LMNA_HUMAN antibody|LMNC antibody|NY REN 32 antigen antibody|Prelamin A/C antibody|PRO1 antibody|Progeria 1(Hutchinson Gilford Type) antibody|Renal carcinoma antigen NY REN 32 antibody|Renal carcinoma antigen NY-REN-32 antibody|Renal carcinoma antigen NYREN32 antibody
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Handling References for anti-Lamin A/C antibody (ABIN3043695) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details References for anti-Lamin A/C antibody (ABIN3043695) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-Lamin A/C antibody (ABIN3043695)Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling Images back to top
|Product cited in:||
Liu, Ma, Wu, Li, Zhang, Han, Wang, Reindl, Wu, Ma: "Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-?B signaling." in: BMC cancer, Vol. 14, pp. 686, 2014
Yang, Liu, Zhu, Zhang, Xi, Hu, He, Jiang: "Human peripheral nerve-derived scaffold for tissue-engineered nerve grafts: histology and biocompatibility analysis." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 96, Issue 1, pp. 25-33, 2010
ImagesProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling References for anti-Lamin A/C antibody (ABIN3043695) back to top