anti-Human Lamin A/C antibody for Western Blotting

Recommended Lamin A/C Antibody (supplied by: Log in to see )

Antigen
Lamin A/C (LMNA) Antibodies
  • CDCD1
  • CDDC
  • CMD1A
  • CMT2B1
  • EMD2
  • FPL
  • FPLD
  • FPLD2
  • HGPS
  • IDC
  • LDP1
  • LFP
  • LGMD1B
  • LMN1
  • LMNC
  • LMNL1
  • PRO1
  • Dhe
  • 2459
  • 74/76
  • CG6944
  • D5
  • DM[[O]]
  • Dm
  • Dm(0)
  • Dm0
  • Dm1
  • Dm2
  • DmLamin
  • DmO
  • Dm[[0]]
  • Dm[[1]]
  • Dm[[2]]
  • Dm[[mit]]
  • Dm[[o]]
  • Dmel\\CG6944
  • Dmo
  • LAM
  • Lam Dm[[0]]
  • Lam(Dm0)
  • LamDm[[0]]
  • LamDm[[o]]
  • Lam[[Dm0]]
  • Lamin Dm[[0]]
  • jf27
  • l(2)04643
  • l(2)25Ec
  • l(2)gdh-7
  • l(2)gdh7
  • l(2)jf27
  • lam
  • lamDm0
  • lamDm[[0]]
  • lamin
  • lamin Dm0
  • lamin Dm[[0]]
  • misg
  • nlam
  • cb948
  • fk66d12
  • wu:fk66d12
  • lmna-A
  • fpl
  • idc
  • lfp
  • cddc
  • emd2
  • fpld
  • hgps
  • ldp1
  • lmn1
  • lmnc
  • pro1
  • cdcd1
  • cmd1a
  • cmt2b1
  • lgmd1b
  • Lamin-A
  • lamin-L(III)-like
  • lamin A/C
  • lamin A
  • Lamin
  • lamin A/C L homeolog
  • lamin-L(III)-like
  • LMNA
  • Lmna
  • Lam
  • lmna
  • lmna.L
  • LMINA
Reactivity
Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Dog (Canine)
444
271
234
98
52
49
25
22
17
10
10
9
5
5
4
4
3
3
2
2
2
1
1
1
1
1
Host
Mouse
237
215
5
3
Clonality
Monoclonal
Conjugate
This Lamin A/C antibody is un-conjugated
13
12
10
7
7
6
6
6
6
6
6
6
5
5
5
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
410
204
198
154
120
112
76
71
54
17
15
11
2
2
1
1
1
Options
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Catalog No. ABIN335389
$ 383.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.056355 ABIN3020785 IHC WB Rabbit IgG Log in to see Polyclonal 0
32.056355 ABIN2854942 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
32.056355 ABIN2856820 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
32.056355 ABIN2854943 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
30.556355 ABIN969260 IHC ELISA WB Mouse IgG1 Log in to see 4-00E-07 1
23.752602 ABIN335390 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see 131C3 3
21.303753 ABIN3043295 ICC IHC (p) WB Rabbit IgG AA 481-646 Log in to see Polyclonal 3
20.056355 ABIN3185344 ELISA IHC WB Rabbit IgG Ser78 Log in to see Polyclonal 0
20.056355 ABIN3185343 ELISA IF IHC WB Rabbit IgG Ser778 Log in to see Polyclonal 0
20.056355 ABIN2017592 FACS WB Rabbit IgG1 AA 400-450 Log in to see Polyclonal 0
20.056355 ABIN6254037 IF WB Mouse IgG1 Log in to see 4C4 0
19.803753 ABIN6256311 ELISA ICC IF IHC WB Rabbit IgG pSer392 Log in to see Polyclonal 0
19.803753 ABIN2988538 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
19.803753 ABIN1873552 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
18.303753 ABIN3043695 ICC IHC (p) WB Rabbit IgG AA 455-469, C-Term Log in to see Polyclonal 3
17.752602 ABIN966489 IHC ELISA WB Mouse IgG1 Log in to see 4E7 2
17.056355 ABIN3181809 ELISA IHC WB Rabbit IgG Asp230, cleaved Log in to see Polyclonal 0
17.056355 ABIN3181810 ELISA IHC WB Rabbit IgG Asn231, cleaved Log in to see Polyclonal 0
17.056355 ABIN6254178 WB Goat IgG C-Term Log in to see Polyclonal 0
17.056355 ABIN6136134 WB Rabbit pSer22 Log in to see Polyclonal 0

General

Antigen Lamin A/C (LMNA) Antibodies
Reactivity Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Dog (Canine)
(444), (271), (234), (98), (52), (49), (25), (22), (17), (10), (10), (9), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(237), (215), (5), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin A/C antibody is un-conjugated
(13), (12), (10), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
(410), (204), (198), (154), (120), (112), (76), (71), (54), (17), (15), (11), (2), (2), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-Lamin A/C Antibody

Target Details Lamin A/C Application Details Handling References for anti-Lamin A/C antibody (ABIN335389) Images
Specificity Human, rat, mouse, bovine, dog.
Purification Purified
Immunogen 133A2 is a mouse monoclonal IgG3/kappa antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.
Clone 133A2
Isotype IgG3
Plasmids, Primers & others

Target Details Lamin A/C

Product Details anti-Lamin A/C Antibody Application Details Handling References for anti-Lamin A/C antibody (ABIN335389) Images back to top
Antigen
Alternative Name Lamin A (LMNA Antibody Abstract)
Background Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.
Pathways Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus

Application Details

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Handling References for anti-Lamin A/C antibody (ABIN335389) Images back to top
Application Notes 133A2 recognizes an epitope located between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A. 133A2 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details References for anti-Lamin A/C antibody (ABIN335389) Images back to top
Storage 4 °C

References for anti-Lamin A/C antibody (ABIN335389)

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling Images back to top
Product cited in:

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, 2003

Broers, Machiels, van Eys, Kuijpers, Manders, van Driel, Ramaekers: "Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins." in: Journal of cell science, Vol. 112 ( Pt 20), pp. 3463-75, 2000

Neri, Raymond, Giordano, Borgatti, Marchisio, Capitani, Martelli: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996

Hozák, Sasseville, Raymond, Cook: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995

Images

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling References for anti-Lamin A/C antibody (ABIN335389) back to top
Supplier Images
 image for anti-Lamin A/C (LMNA) antibody (ABIN335389) anti-Lamin A/C (LMNA) antibody
 image for anti-Lamin A/C (LMNA) antibody (ABIN335389) Immunocytochemical staining of fiboblasts showing nuclear lamina