anti-Zebrafish (Danio rerio) Lamin B1 antibody for Immunocytochemistry

Recommended Lamin B1 Antibody (supplied by: Log in to see )

Antigen
Lamin B1 (LMNB1) Antibodies
  • MGC52550
  • Lamin-L(I)
  • lamin-b
  • lmn
  • lmn2
  • lmnb
  • LMNB1
  • ADLD
  • LMN
  • LMN2
  • LMNB
  • fc06g01
  • wu:fc06g01
  • lamin B1 L homeolog
  • lamin B1
  • lmnb1.L
  • lmnb1
  • LMNB1
  • Lmnb1
Reactivity
Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Rabbit, Dog (Canine), Sheep (Ovine), Zebrafish (Danio rerio)
167
78
67
12
10
10
5
5
4
3
3
3
2
2
1
1
1
Host
Mouse
100
42
31
Clonality
Monoclonal
Conjugate
This Lamin B1 antibody is un-conjugated
3
3
3
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
164
78
62
34
32
27
15
13
11
7
5
2
1
1
1
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Catalog No. ABIN335391
$ 376.36
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN108542 DB ELISA FACS ICC IF IHC IP WB Mouse IgG1 kappa C-Term Log in to see 119D5-F1 12
1 ABIN2963329 ICC IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 119D5-F1 0

General

Antigen Lamin B1 (LMNB1) Antibodies
Reactivity Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Rabbit, Dog (Canine), Sheep (Ovine), Zebrafish (Danio rerio)
(167), (78), (67), (12), (10), (10), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(100), (42), (31)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin B1 antibody is un-conjugated
(3), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(164), (78), (62), (34), (32), (27), (15), (13), (11), (7), (5), (2), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-Lamin B1 Antibody

Target Details Lamin B1 Application Details Handling References for anti-Lamin B1 antibody (ABIN335391) Images
Specificity Human, rat, mouse, bovine, rabbit, dog, sheep.
Purification Purified
Immunogen 119D5-F1 is a mouse monoclonal IgG1/kappa antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.
Clone 119D5-F1
Isotype IgG1
Plasmids, Primers & others

Target Details Lamin B1

Product Details anti-Lamin B1 Antibody Application Details Handling References for anti-Lamin B1 antibody (ABIN335391) Images back to top
Antigen
Alternative Name Lamin B1 (LMNB1 Antibody Abstract)
Background Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Handling References for anti-Lamin B1 antibody (ABIN335391) Images back to top
Application Notes 119D5-F1 reacts with an epitope located C-terminal of residue 231 in lamin B1. 119D5-F1 is suitable for immunocytochemistry, immunoblotting, ELISA and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details References for anti-Lamin B1 antibody (ABIN335391) Images back to top
Storage 4 °C

References for anti-Lamin B1 antibody (ABIN335391)

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details Handling Images back to top
Product cited in:

Broers, Ramaekers, Bonne, Yaou, Hutchison: "Nuclear lamins: laminopathies and their role in premature ageing." in: Physiological reviews, Vol. 86, Issue 3, pp. 967-1008, 2006

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, 2003

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, 1996

Images

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Supplier Images
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Lamin B1 (LMNB1) antibody (ABIN335391) Immunohistochemistry on frozen sections of swine liver showing nuclear lamina stainin...