anti-Human NME1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended NME1 Antibody (supplied by: Log in to see )

Antigen
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
  • NDK I
  • NDPK I
  • NME1
  • NME1-NME2
  • AWD
  • GAAD
  • NB
  • NBS
  • NDKA
  • NDPK-A
  • NDPKA
  • NM23
  • NM23-H1
  • AL024257
  • NM23-M1
  • NM23A
  • ndpk-b
  • ndpkb
  • nm23-h2
  • nm23b
  • nm23ndk
  • nme1
  • puf
  • nm23ndk-a
  • nme2
  • NDPK-Z1
  • NM23-B
  • NM23-Z1
  • ndpkz1
  • nme2b1
  • NM23-C1
  • NBR-A
  • NBR-B
  • NME1-1
  • T30A10.80
  • T30A10_80
  • CHH
  • RMRPR
  • RRP2
  • nucleoside diphosphate kinase
  • non-metastatic cells 1, protein (NM23A) expressed in
  • NME/NM23 nucleoside diphosphate kinase 1
  • NME/NM23 nucleoside diphosphate kinase 2
  • NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1
  • nucleoside diphosphate kinase 1
  • RNA component of mitochondrial RNA processing endoribonuclease
  • LOC547870
  • NME1
  • Nme1
  • nme2-b
  • nme2-a
  • nme2b.1
  • NDPK1
  • RMRP
Reactivity
Human, Mouse (Murine), Rat (Rattus)
292
53
50
14
9
8
6
4
4
3
3
2
2
1
1
Host
Rabbit
145
142
6
2
2
Clonality
Polyclonal
Conjugate
This NME1 antibody is un-conjugated
12
12
8
7
6
6
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
250
125
88
63
40
15
13
12
10
7
6
2
2
1
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Catalog No. ABIN498563
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.588177 ABIN440929 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
13.588177 ABIN4340146 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 4B2
13.588177 ABIN4912425 IHC (p) WB Mouse Log in to see 1172CT2-4-1-1
1 ABIN3044197 ICC IHC (p) WB Rabbit IgG AA 137-152, C-Term Log in to see Polyclonal
1 ABIN392655 IF IHC (p) WB Rabbit Ig AA 25-54, N-Term Log in to see Polyclonal 2
1 ABIN562004 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 43-152, partial Log in to see 1D7 1
1 ABIN5552299 EIA IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
1 ABIN604776 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN782418 EIA IF IHC (p) IP WB Mouse IgG1 AA 43-153 Log in to see 1D7
1 ABIN1108395 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4B2 1
1 ABIN446993 IHC (p) IHC WB Rabbit Log in to see Polyclonal
1 ABIN1998965 IHC (p) Rabbit IgG AA 2-152 Log in to see 084
1 ABIN1499746 IHC (p) WB Mouse IgG2a Log in to see 2A5
1 ABIN1499748 IHC (p) WB Mouse IgG2a Log in to see 3F2
1 ABIN1499749 IHC (p) WB Mouse IgG1 Log in to see 1C12
1 ABIN1499750 IHC (p) WB Mouse IgG2a Log in to see 7F6
1 ABIN1499757 IHC (p) WB Mouse IgG2b Log in to see 22F10
1 ABIN1499762 IHC (p) WB Mouse IgG1 Log in to see 1C11
1 ABIN1499763 IHC (p) WB Mouse IgG2a Log in to see 7F4
1 ABIN1499761 IHC (p) WB Mouse IgG2b Log in to see 24A2

General

Antigen Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(292), (53), (50), (14), (9), (8), (6), (4), (4), (3), (3), (2), (2), (1), (1)
Host Rabbit
(145), (142), (6), (2), (2)
Clonality
Polyclonal
Conjugate This NME1 antibody is un-conjugated
(12), (12), (8), (7), (6), (6)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(250), (125), (88), (63), (40), (15), (13), (12), (10), (7), (6), (2), (2), (1)
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Product Details anti-NME1 Antibody

Target Details NME1 Application Details Handling Images
Specificity This antibody detects endogenous levels of NM23-H1 protein. (region surrounding Tyr52)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details NME1

Product Details anti-NME1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NDP Kinase A (NME1 Antibody Abstract)
Background The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sublines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.Synonyms: GAAD, Granzyme A-activated DNase, NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, nm23-H1
Molecular Weight approx. 20 kDa
Gene ID 4830
NCBI Accession NP_000260
UniProt P15531
Research Area Kinases/Phosphatases, Cancer, Transcription Factors
Pathways Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process

Application Details

Product Details anti-NME1 Antibody Target Details NME1 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NME1 Antibody Target Details NME1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) antibody (ABIN498563) Immunohistochemistry (IHC) analyzes of NM23-H1 antibody in paraffin-embedded h...
Western Blotting (WB) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) antibody (ABIN498563) Western blot (WB) analysis of NM23-H1 antibody in extracts from HeLa cells.