anti-Rat (Rattus) NME1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended NME1 Antibody (supplied by: Log in to see )

Antigen
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
  • AWD
  • GAAD
  • NB
  • NBS
  • NDKA
  • NDPK-A
  • NDPKA
  • NM23
  • NM23-H1
  • AL024257
  • NM23-M1
  • NM23A
  • NDPK-Z1
  • NM23-B
  • NM23-Z1
  • ndpkz1
  • nm23b
  • nme1
  • nme2
  • nme2b1
  • NME1
  • NME1-NME2
  • NM23-C1
  • ndpk-b
  • ndpkb
  • nm23-h2
  • nm23ndk
  • puf
  • NBR-A
  • NBR-B
  • NME1-1
  • nm23ndk-a
  • NDK I
  • NDPK I
  • T30A10.80
  • T30A10_80
  • NDP kinase I
  • NME/NM23 nucleoside diphosphate kinase 1
  • NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1
  • non-metastatic cells 1, protein (NM23A) expressed in
  • NME/NM23 nucleoside diphosphate kinase 2 S homeolog
  • NME/NM23 nucleoside diphosphate kinase 2 L homeolog
  • nucleoside diphosphate kinase
  • nucleoside diphosphate kinase 1
  • NME1
  • Nme1
  • nme2b.1
  • nme2.S
  • nme2.L
  • LOC547870
  • NDPK1
  • LOC107790563
Epitope
AA 137-152, C-Term
23
15
13
12
12
11
8
8
8
8
8
6
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
296
57
57
12
8
7
5
4
4
3
2
2
2
1
1
Host
Rabbit
151
142
7
2
2
Clonality
Polyclonal
Conjugate
This NME1 antibody is un-conjugated
12
12
8
7
6
6
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
266
134
128
67
39
18
14
12
10
8
7
2
2
2
1
Options
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Catalog No. ABIN3044197
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.075163 ABIN4902019 IHC (p) Rabbit IgG Log in to see Polyclonal 0
11.426572 ABIN498563 IHC (p) WB Rabbit Log in to see Polyclonal 0
11.426572 ABIN4912425 IHC (p) WB Mouse Log in to see 1172CT2-4-1-1 0
10 ABIN604776 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
10 ABIN3032024 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN5961750 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2887782 ELISA IHC IHC (p) WB Rabbit IgG AA 3-52 Log in to see Polyclonal 0
1 ABIN2892931 IHC IHC (p) WB Rabbit Tyr52 Log in to see Polyclonal 0
1 ABIN2894316 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN5961751 IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2726968 IHC (p) Mouse IgG2a Log in to see UMAB91 0
1 ABIN2726969 IHC (p) Mouse IgG1 Log in to see UMAB92 0
1 ABIN2726970 IHC (p) Mouse IgG1 Log in to see UMAB93 0
1 ABIN2726971 IHC (p) Mouse IgG1 Log in to see UMAB94 0
1 ABIN2726975 IHC (p) Mouse IgG1 Log in to see UMAB94 0
1 ABIN2726974 IHC (p) Mouse IgG1 Log in to see UMAB93 0
1 ABIN2726973 IHC (p) Mouse IgG1 Log in to see UMAB92 0
1 ABIN2726972 IHC (p) Mouse IgG2a Log in to see UMAB91 0

General

Antigen Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
Epitope AA 137-152, C-Term
(23), (15), (13), (12), (12), (11), (8), (8), (8), (8), (8), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(296), (57), (57), (12), (8), (7), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(151), (142), (7), (2), (2)
Clonality
Polyclonal
Conjugate This NME1 antibody is un-conjugated
(12), (12), (8), (7), (6), (6)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(266), (134), (128), (67), (39), (18), (14), (12), (10), (8), (7), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-NME1 Antibody

Target Details NME1 Application Details Handling References for anti-NME1 antibody (ABIN3044197) Images
Purpose Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase A(NME1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Sequence EELVDYTSCA QNWIYE
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase A(NME1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: NME/NM23 nucleoside diphosphate kinase 1
Protein Name: Nucleoside diphosphate kinase A(NDK A)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human NM23A(137-152aa EELVDYTSCAQNWIYE), different from the related mouse sequence by two amino acids and from rat sequence by one amino acid.
Isotype IgG
Plasmids, Primers & others

Target Details NME1

Product Details anti-NME1 Antibody Application Details Handling References for anti-NME1 antibody (ABIN3044197) Images back to top
Antigen
Alternative Name NME1 (NME1 Antibody Abstract)
Background NME1(NME/NM23 nucleoside diphosphate kinase 1) also called non-metastatic cells 1, protein(NM23A) expressed in,NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al.(2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.

Synonyms: AWD antibody|AWD, drosophila, homolog of antibody|GAAD antibody|Granzyme A activated DNase antibody|Granzyme A-activated DNase antibody|GZMA activated DNase antibody|Metastasis inhibition factor NM23 antibody|NB antibody|NBS antibody|NDK A antibody|NDKA antibody|NDKA_HUMAN antibody|NDP kinase A antibody|NDPK-A antibody|NDPKA antibody|NM23 antibody|NM23 long variant, included antibody|nm23-H1 antibody|NM23-M1 antibody|NM23H1B, included antibody|NME/NM23 nucleoside diphosphate kinase 1 antibody|Nme1 antibody|NME1-NME2 spliced read-through transcript, included antibody|Non-metastatic cells 1, protein(NM23A) expressed in antibody|Nonmetastatic cells 1, protein expressed in antibody|Nonmetastatic protein 23 antibody|Nonmetastatic protein 23, homolog 1 antibody|Nucleoside diphosphate kinase A antibody|Tumor metastatic process-associated protein antibody
UniProt P15531
Pathways Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process

Application Details

Product Details anti-NME1 Antibody Target Details NME1 Handling References for anti-NME1 antibody (ABIN3044197) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-NME1 Antibody Target Details NME1 Application Details References for anti-NME1 antibody (ABIN3044197) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-NME1 antibody (ABIN3044197)

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling Images back to top
Product cited in:

Jin, Dai: "Mutation of the nm23-H1 gene has a non-dominant role in colorectal adenocarcinoma." in: Molecular and clinical oncology, Vol. 5, Issue 1, pp. 107-110, 2016

Images

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling References for anti-NME1 antibody (ABIN3044197) back to top
Supplier Images
Western Blotting (WB) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibody (ABIN3044197) anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibody
Immunohistochemistry (IHC) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibody (ABIN3044197) Anti-NM23A antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibody (ABIN3044197) Anti-NM23A antibody, IHC(P) IHC(P): Rat Cerebellum Tissue
Flow Cytometry (FACS) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibody (ABIN3044197) Flow Cytometry analysis of Hela cells using anti-NME1 antibody . Overlay histogram sh...