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|Antigen||X-Linked Inhibitor of Apoptosis (XIAP) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This XIAP antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-XIAP AntibodyTarget Details XIAP Application Details Handling References for anti-XIAP antibody (ABIN358604) Images
|Specificity||This antibody reacts to XIAP (BIRC4).|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BIRC4.|
Target Details XIAPProduct Details anti-XIAP Antibody Application Details Handling References for anti-XIAP antibody (ABIN358604) Images back to top
|Alternative Name||IAP3 / BIRC4 (XIAP Antibody Abstract)|
|Background||BIRC4 is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Similar to API1, BIRC4 inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.Synonyms: API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, HILP, Inhibitor of apoptosis protein 3, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP|
|Gene ID||331, 9606|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis|
Application DetailsProduct Details anti-XIAP Antibody Target Details XIAP Handling References for anti-XIAP antibody (ABIN358604) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-XIAP Antibody Target Details XIAP Application Details References for anti-XIAP antibody (ABIN358604) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-XIAP antibody (ABIN358604)Product Details anti-XIAP Antibody Target Details XIAP Application Details Handling Images back to top
Srinivasula, Gupta, Datta, Zhang, Hegde, Cheong, Fernandes-Alnemri, Alnemri: "Inhibitor of apoptosis proteins are substrates for the mitochondrial serine protease Omi/HtrA2." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 31469-72, 2003
Miranda, Dyer, Grandis, Johnson: "Differential activation of apoptosis regulatory pathways during monocytic vs granulocytic differentiation: a requirement for Bcl-X(L)and XIAP in the prolonged survival of monocytic cells." in: Leukemia, Vol. 17, Issue 2, pp. 390-400, 2003
Ahn, Choe, Kwon: "The gene expression profile of human umbilical vein endothelial cells stimulated with lipopolysaccharide using cDNA microarray analysis." in: International journal of molecular medicine, Vol. 12, Issue 2, pp. 231-6, 2003
ImagesProduct Details anti-XIAP Antibody Target Details XIAP Application Details Handling References for anti-XIAP antibody (ABIN358604) back to top