anti-Human XIAP antibody for Western Blotting

Recommended XIAP Antibody (supplied by: Log in to see )

Antigen
X-Linked Inhibitor of Apoptosis (XIAP) Antibodies
  • API3
  • BIRC4
  • IAP-3
  • ILP1
  • MIHA
  • XLP2
  • hIAP-3
  • hIAP3
  • api3
  • birc4
  • ilp1
  • miha
  • xlp2
  • xxiap
  • 1110015C02Rik
  • Aipa
  • Api3
  • Birc4
  • IAP3
  • ILP-1
  • mXIAP
  • riap3
  • fb73d01
  • wu:fb73d01
  • zgc:91873
  • BIRC8
  • X-linked inhibitor of apoptosis
  • X-linked inhibitor of apoptosis L homeolog
  • XIAP
  • xiap
  • xiap.L
  • Xiap
Epitope
AA 14-34, N-Term
20
18
15
9
9
8
8
6
5
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
184
94
61
7
5
5
2
Host
Rabbit
158
26
1
Clonality
Polyclonal
Conjugate
This XIAP antibody is un-conjugated
6
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
163
73
54
45
28
23
13
13
9
9
3
3
2
1
1
Options
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Catalog No. ABIN3044425
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.252573 ABIN968133 IF IP IHC WB Mouse IgG1 AA 268-426 Log in to see 28-hILP-XIAP 6
3.252573 ABIN2854771 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.252573 ABIN4366395 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 1
3.252573 ABIN2707256 IHC WB Rabbit Center Log in to see Polyclonal 0
3.252573 ABIN968132 IF IP IHC WB Mouse IgG1 AA 268-426 Log in to see 28-hILP-XIAP 6
3.252573 ABIN222972 WB Rabbit IgG Log in to see Polyclonal 0
3.252573 ABIN968163 IF IP IHC WB Mouse IgG1 AA 268-426 Log in to see 48-hILP-XIAP 5
3.252573 ABIN968164 IF IP IHC WB Mouse IgG1 AA 268-426 Log in to see 48-hILP-XIAP 5
3.252573 ABIN390070 IHC (p) WB Rabbit Ig Fraction AA 1-30, N-Term Log in to see Polyclonal 2
3.252573 ABIN1872424 ICC IHC WB Rabbit IgG AA 1-497 Log in to see Polyclonal 0
3.252573 ABIN947569 IF IP WB Rabbit AA 1-497, full length Log in to see Polyclonal 0
3.252573 ABIN269889 IHC IP WB Rabbit IgG Log in to see Polyclonal 0
3.252573 ABIN615851 IF IHC (p) WB Rabbit AA 75-226 Log in to see Polyclonal 0
3.252573 ABIN2560797 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.252573 ABIN513401 IF PLA WB Rabbit AA 1-497, full length Log in to see Polyclonal 0
3.252573 ABIN1875360 WB Rabbit IgG Log in to see Polyclonal 0
3.252573 ABIN2737825 ICC WB Rabbit IgG Log in to see Polyclonal 0
3.252573 ABIN3187502 ELISA IHC WB Rabbit IgG Ser144 Log in to see Polyclonal 0
3.252573 ABIN3182566 ELISA IHC WB Rabbit IgG pSer87 Log in to see Polyclonal 0
3.252573 ABIN513399 WB Mouse AA 1-497, full length Log in to see Polyclonal 0

General

Antigen X-Linked Inhibitor of Apoptosis (XIAP) Antibodies
Epitope AA 14-34, N-Term
(20), (18), (15), (9), (9), (8), (8), (6), (5), (5), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(184), (94), (61), (7), (5), (5), (2)
Host Rabbit
(158), (26), (1)
Clonality
Polyclonal
Conjugate This XIAP antibody is un-conjugated
(6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(163), (73), (54), (45), (28), (23), (13), (13), (9), (9), (3), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-XIAP Antibody

Target Details XIAP Application Details Handling References for anti-XIAP antibody (ABIN3044425) Images
Purpose Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase XIAP(XIAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Sequence ADINKEEEFV EEFNRLKTFA N
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase XIAP(XIAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: X-linked inhibitor of apoptosis
Protein Name: E3 ubiquitin-protein ligase XIAP
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human XIAP(14-34aa ADINKEEEFVEEFNRLKTFAN), different from the related mouse sequence by two amino acids.
Isotype IgG

Target Details XIAP

Product Details anti-XIAP Antibody Application Details Handling References for anti-XIAP antibody (ABIN3044425) Images back to top
Antigen
Alternative Name XIAP (XIAP Antibody Abstract)
Background BIRC4, baculoviral IAP repeat-containing protein 4 is also known as XIAP(X-linked inhibitor of apoptosis protein). The BIRC4 gene comprises 6 exons. This gene is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.

Synonyms: AP 13 antibody|API3 antibody|Apoptosis Inhibitor 3 antibody|Baculoviral IAP repeat containing 4 antibody|Baculoviral IAP Repeat Containing Protein 4 antibody|Baculoviral IAP repeat-containing protein 4 antibody|BIRC 4 antibody|BIRC4 antibody|E3 ubiquitin-protein ligase XIAP antibody|hIAP-3 antibody|hIAP3 antibody|HILP antibody|IAP 3 antibody|IAP like protein antibody|IAP-3 antibody|IAP-like protein antibody|IAP3 antibody|ILP 1 antibody|ILP antibody|ILP1 antibody|Inhibitor of apoptosis protein 3 antibody|Inhibitor of Apoptosis X Linked antibody|Mammalian IAP Homologue A antibody|MIHA antibody|X linked IAP antibody|X linked inhibitor of apoptosis antibody|X linked inhibitor of apoptosis protein antibody|X-linked IAP antibody|X-linked inhibitor of apoptosis protein antibody|Xiap antibody|XIAP_HUMAN antibody|XLP2 antibody
UniProt P98170
Pathways Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis

Application Details

Product Details anti-XIAP Antibody Target Details XIAP Handling References for anti-XIAP antibody (ABIN3044425) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-XIAP Antibody Target Details XIAP Application Details References for anti-XIAP antibody (ABIN3044425) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-XIAP antibody (ABIN3044425)

Product Details anti-XIAP Antibody Target Details XIAP Application Details Handling Images back to top
Product cited in:

Chen, Mao, Qiu, Liu: "Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage." in: Neural regeneration research, Vol. 10, Issue 3, pp. 425-31, 2015

Li, Zhang, Fang, Yan, Zhao, Feng, Ma, Ye: "Aspirin overcomes Navitoclax-resistance in hepatocellular carcinoma cells through suppression of Mcl-1." in: Biochemical and biophysical research communications, Vol. 434, Issue 4, pp. 809-14, 2013

Wu, Xi, Guo, Liu, Yang, Liu, Dong, Guo: "Exogenous ARC down-regulates caspase-3 expression and inhibits apoptosis of broiler chicken cardiomyocytes exposed to hydrogen peroxide." in: Avian pathology : journal of the W.V.P.A, Vol. 42, Issue 1, pp. 32-7, 2013

Images

Product Details anti-XIAP Antibody Target Details XIAP Application Details Handling References for anti-XIAP antibody (ABIN3044425) back to top
Supplier Images
Western Blotting (WB) image for anti-X-Linked Inhibitor of Apoptosis (XIAP) (AA 14-34), (N-Term) antibody (ABIN3044425) anti-X-Linked Inhibitor of Apoptosis (XIAP) (AA 14-34), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-X-Linked Inhibitor of Apoptosis (XIAP) (AA 14-34), (N-Term) antibody (ABIN3044425) Anti-XIAP antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue