anti-Human YWHAQ antibody for Western Blotting

Recommended YWHAQ Antibody (supplied by: Log in to see )

Antigen
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies
  • 14-3-3
  • 14-3-3t
  • 1c5
  • hs1
  • 1C5
  • HS1
  • 2700028P07Rik
  • AA409740
  • AU021156
  • R74690
  • ywhaq
  • ywhaq2
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
  • 14-3-3 protein theta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a
  • ywhaq
  • YWHAQ
  • Ywhaq
  • ywhaq.S
  • ywhaqa
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
176
119
107
39
25
24
17
14
14
12
10
10
6
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
Host
Mouse
128
35
17
7
1
Clonality
Monoclonal
Conjugate
This YWHAQ antibody is un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
159
66
60
40
35
26
22
8
5
4
3
3
1
1
Options
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Catalog No. ABIN2688836
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9820433 ABIN2792144 IHC WB Rabbit N-Term Log in to see Polyclonal 2
2.9820433 ABIN2705348 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
2.9820433 ABIN2704426 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
2.9820433 ABIN449751 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 1-245 Log in to see 1A1 0
2.9820433 ABIN1534236 ELISA WB Rabbit IgG AA 41-90 Log in to see Polyclonal 0
2.9820433 ABIN336110 EIA IHC (p) WB Mouse IgG1 AA 1-245, N-Term Log in to see AT1A1 2
2.9820433 ABIN1532491 IF ELISA WB Rabbit IgG AA 196-245 Log in to see Polyclonal 0
2.9820433 ABIN1531476 IF IHC ELISA WB Rabbit IgG AA 196-245, pSer232 Log in to see Polyclonal 0
2.9820433 ABIN2688838 WB Mouse IgG1, kappa Log in to see C23 2
2.9820433 ABIN2855319 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
2.9820433 ABIN441537 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
2.9820433 ABIN3183083 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
2.9820433 ABIN3182780 ELISA IF IHC WB Rabbit IgG pSer232 Log in to see Polyclonal 0
2.9820433 ABIN3183084 ELISA IF IHC WB Rabbit IgG Tyr588, Tyr596 Log in to see Polyclonal 0
2.9820433 ABIN264385 EIA WB Mouse IgG1 Log in to see 3B9 1
2.9820433 ABIN451588 ELISA WB Goat Log in to see Polyclonal 1
2.9820433 ABIN4276646 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 0
2.9820433 ABIN1875379 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
2.9820433 ABIN2465330 ELISA WB Goat Internal Region Log in to see Polyclonal 0
2.9820433 ABIN1583873 ELISA IHC WB Rabbit N-Term Log in to see Polyclonal 0

General

Antigen tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(176), (119), (107), (39), (25), (24), (17), (14), (14), (12), (10), (10), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(128), (35), (17), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This YWHAQ antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(159), (66), (60), (40), (35), (26), (22), (8), (5), (4), (3), (3), (1), (1)
Pubmed 5 references available
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Product Details anti-YWHAQ Antibody

Target Details YWHAQ Application Details Handling References for anti-YWHAQ antibody (ABIN2688836) Images
Characteristics The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ. Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- 14-3-3 antibody. Immunofluorescence staining of SKN cells (Human neuroblastoma).

BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3 - Purified - Clone 12/14-3-3 - Isotype Mouse IgM - Reactivity Hu, Chick, Dog, Frog, Ms, Rat - 150 μg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human 14-3-3
Clone 12-14-3
Isotype IgM

Target Details YWHAQ

Product Details anti-YWHAQ Antibody Application Details Handling References for anti-YWHAQ antibody (ABIN2688836) Images back to top
Antigen
Alternative Name 14-3-3 (YWHAQ Antibody Abstract)
Molecular Weight 29 kDa
Pathways Apoptosis, Myometrial Relaxation and Contraction

Application Details

Product Details anti-YWHAQ Antibody Target Details YWHAQ Handling References for anti-YWHAQ antibody (ABIN2688836) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details References for anti-YWHAQ antibody (ABIN2688836) Images back to top
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-YWHAQ antibody (ABIN2688836)

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling Images back to top
Product cited in:

Mamane, Grandvaux, Hernandez, Sharma, Innocente, Lee, Azimi, Lin, Hiscott: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." in: Oncogene, Vol. 21, Issue 44, pp. 6751-65, 2002

Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: "Bcl-2 targets protein phosphatase 1 alpha to Bad." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 12, pp. 7345-52, 2001

Ostrerova, Petrucelli, Farrer, Mehta, Choi, Hardy, Wolozin: "alpha-Synuclein shares physical and functional homology with 14-3-3 proteins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5782-91, 1999

Conklin, Galaktionov, Beach: "14-3-3 proteins associate with cdc25 phosphatases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7892-6, 1995

Freed, Symons, Macdonald, McCormick, Ruggieri: "Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1713-6, 1994

Images

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling References for anti-YWHAQ antibody (ABIN2688836) back to top
Supplier Images
Western Blotting (WB) image for anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) antibody (ABIN2688836) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) antibody