anti-Chicken YWHAZ antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended YWHAZ Antibody

Antigen
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) Antibodies
  • 14-3-3-zeta
  • KCIP-1
  • YWHAD
  • 14-3-3zeta
  • Ywhaz
  • ACYPI003154
  • 14-3-3z
  • kcip-1
  • ywhaq
  • 1433z
  • ywhaz
  • ywhazb
  • 1110013I11Rik
  • AI596267
  • AL022924
  • AU020854
  • ywhaza
  • fb14h09
  • wu:fb05g08
  • wu:fb14h09
  • ywhai
  • zgc:55807
  • 14-3-3
  • 14-3-3 zeta
  • 14-3-3ZETA
  • 14-3-3leo
  • 2G1
  • 4-3-3 zeta
  • 5.11
  • 549
  • BEST:GH05075
  • CG17870
  • D14-3-3
  • D14-3-3zeta
  • Dmel\\CG17870
  • K
  • LEO
  • Leo
  • PAR-5
  • PAR5
  • Par-5
  • d14-3-3zeta
  • l(2)07103
  • l(2)46CFe
  • l(2)46Ee
  • leo
  • par-5
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
  • 14-3-3 protein zeta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog
  • 14-3-3 protein zeta/delta pseudogene
  • CG17870 gene product from transcript CG17870-RE
  • YWHAZ
  • 14-3-3zeta
  • ywhaz
  • 1433z
  • ywhaz.L
  • Ywhaz
  • ywhaz.S
  • LOC100855903
Epitope
N-Term, acetylated
60
12
12
10
10
10
7
7
7
6
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
250
177
146
24
23
19
19
11
7
7
6
6
4
3
2
2
1
1
Host
Rabbit
242
10
Clonality
Polyclonal
Conjugate
This YWHAZ antibody is un-conjugated
10
6
5
4
4
4
4
4
4
3
3
3
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
209
126
88
61
53
24
14
13
12
6
3
2
Options

Available images

Catalog No. ABIN318724
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
7 ABIN6747116 IHC IHC (p) WB Rabbit C-Term Polyclonal 0
1 ABIN609110 ELISA IHC IHC (p) WB Rabbit IgG AA 1-10 Polyclonal 0
1 ABIN2629952 ICC IF IHC IHC (p) WB Rabbit C-Term Polyclonal 0
1 ABIN2629950 ICC IF IHC IHC (p) IP WB Rabbit Internal Region Polyclonal 0
1 ABIN2629949 ICC IF IHC IHC (p) IP WB Rabbit pSer58 Polyclonal 0
1 ABIN576198 ELISA IHC IHC (p) WB Rabbit IgG N-Term, acetylated Polyclonal 0

General

Antigen tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) Antibodies
Epitope N-Term, acetylated
(60), (12), (12), (10), (10), (10), (7), (7), (7), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(250), (177), (146), (24), (23), (19), (19), (11), (7), (7), (6), (6), (4), (3), (2), (2), (1), (1)
Host Rabbit
(242), (10)
Clonality
Polyclonal
Conjugate This YWHAZ antibody is un-conjugated
(10), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(209), (126), (88), (61), (53), (24), (14), (13), (12), (6), (3), (2)
Pubmed 2 references available
Supplier

Product Details anti-YWHAZ Antibody

Target Details YWHAZ Application Details Handling References for anti-YWHAZ antibody (ABIN318724) Images
Sequence Ac.MDKNELVQKA C
Specificity This antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. This antibody may not react with recombinant proteins that are not N-acetylated.
Cross-Reactivity (Details) Species reactivity (expected):Mammals.
Species reactivity (tested):Sheep, Bovine, Chicken, Rat, Human, Mouse.
Purification Serum
Immunogen Synthetic peptide corresponding to acetylated N-terminal sequence of Sheep 14-3-3 zeta.
Isotype IgG
Plasmids, Primers & others

Target Details YWHAZ

Product Details anti-YWHAZ Antibody Application Details Handling References for anti-YWHAZ antibody (ABIN318724) Images back to top
Antigen
Alternative Name 14-3-3 Protein zeta/delta (YWHAZ Antibody Abstract)
Background It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ
Gene ID 9940
UniProt P29361
Pathways Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location

Application Details

Product Details anti-YWHAZ Antibody Target Details YWHAZ Handling References for anti-YWHAZ antibody (ABIN318724) Images back to top
Application Notes Immunohistochemistry on paraffin sections: requires antigen retrieval using heattreatment prior to staining of paraffin sections. ELISA. Western Blot: detects a band of approximately 30 kDa in HEK293 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details References for anti-YWHAZ antibody (ABIN318724) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-YWHAZ antibody (ABIN318724)

Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling Images back to top
Product cited in:

Aitken: "14-3-3 proteins: a historic overview." in: Seminars in cancer biology, Vol. 16, Issue 3, pp. 162-72, 2006

Martin, Patel, Jones, Howell, Robinson, Aitken: "Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein." in: FEBS letters, Vol. 331, Issue 3, pp. 296-303, 1993

Images

Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling References for anti-YWHAZ antibody (ABIN318724) back to top
Supplier Images
 image for anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (acetylated), (N-Term) antibody (ABIN318724) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (acetylated), (N-Term) antibody