Protein G Sepharose Bead
- Immunoprecipitation (IP), Purification (Purif)
- Protein G Sepharose for affinity purification of antibodies.
- High binding capacity = Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
Minimal Leaching of the Ligand
Flow Rate Tested* = 2.07 ml/min.
*Test condition: = Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
Usage = Reusable for up to 10 times without significant loss of binding capacity.
- Protein G-Sepharose beads are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose beads. Protein G (Cat. # ABIN412519) is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G-Sepharose is >20 mg of human or rabbit IgG per ml of wet beads. Protein G-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
- Bead Ligand
- Protein G
- Bead Matrix
- Sepharose beads
- Bead Size
- 90 µm
- Application Notes
- - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
- For Research Use only
- Supplied as 50% slurry in 20 % Ethanol/H2O.
- Handling Advice
- Do not freeze!
- 4 °C
- Expiry Date
- 12 months
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