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Protein L Sepharose Bead

IP, Purif Protein L Sepharose beads 90 µm
Catalog No. ABIN412460
$205.00
Plus shipping costs $45.00
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  • Target
    Reactivity
    Peptostreptococcus magnus
    Application
    Immunoprecipitation (IP), Purification (Purif)
    Purpose
    Purification of monoclonal and polyclonal antibodies, Immunoprecipitation.
    Specificity
    Binding capacity of human IgG is greater than 10 mg/ml of gel, High flow rate, Low falling off of rProteinL, pH stability 2-10.
    Characteristics
    Protein L-Sepharose is prepared by covalently coupling recombinant Protein L (contains fiveIglight chain binding domains, BV catalog # ABIN412523) to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding is generally greater than 10 mg of human IgG per ml of wet gel.
    Bead Ligand
    Protein L
    Bead Matrix
    Sepharose beads
    Bead Size
    90 µm
  • Target
  • Application Notes
    IgG purification procedure eample:
    1. Wash column with ddH2O to remove air bubbles.
    2. fill column with protein L beads.
    3. Wash the column with 5X volume of Binding Buffer.
    4. Dilute serum sample with Binding Buffer (1:1 ratio).
    5. Invert the diluted serum sample to mix well. Make sure no bubbles in the solution.
    6. Pour the solution onto the column.
    7. Collect the solution and repeat step 6 & 7 for 10 times.
    8. Wash the column 5-10 times with the Binding Buffer.
    9. Add Elution Buffer to elute IgG.
    10. Collect the eluent using microcentrifuge tube.
    11. Repeat step 9 & 10 for 10 times.
    12. Assay protein concentration and combine the fractions containing sufficient amount of IgG.

    Buffer example:
    Binding buffer: 0.05 M sodium borate, 0.15 M sodium chloride pH 8.0
    Elution buffer: 0.1 M citric acid, pH 2.75.
    Note: Protein L binds to all IgG subclasses from human, mouse and rat species. It also binds to human, mouse, and rat IgM, IgA, IgE, and IgD, as well as Fab and K light chains. Protein L is also superior for binding to chicken, Hamster and pig IgG.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Supplied as a 50% slurry in 20 % Ethanol/H₂O, >5 mg Protein L/ml of sepharose beads. 
    Handling Advice
    Do not freeze!
    Storage
    4 °C
    Expiry Date
    12 months
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