GFP-Booster (Atto 594)

Details for Product No. ABIN2452219, Supplier: Log in to see
  • green fluorescent protein
  • gfp
Aequorea victoria
Camelid (Camelidae)
Antibody Type
Recombinant Antibody
Atto 594
Fluorescence Microscopy (FM), Immunofluorescence (IF)
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Supplier Product No.
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Purpose With our Booster you reactivate, boost, stabilizate the signals of your fusion proteins.
Brand GFP-Trap®
Specificity GFP-Booster efficiently detects and labels most common GFP derivates. No binding to red fluorescent proteins derived from DsRed can be detected.
  • Enhance, stabilize and reactivate your fl uorescent proteins
  • GFP-Booster highly specifi c for GFP fusion proteins (and derivatives thereof e.g. YFP or Venus)
  • Coupled to bright and photostable chemical dyes from ATTO-TEC
Components GFP-Trap® coupled to fluorescent dye ATTO 594
Antigen Green Fluorescent Protein (GFP)
Alternative Name GFP
Background Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics in living cells. However, photo stability and quantum efficiency of GFP are not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples. In addition, many cell biological methods such as BrdU-staining, EdU-Click-iT™ treatment or Fluorescent In Situ Hybridization result in disruption of the GFP signal.The GFP-Booster_Atto594, a specific GFP-binding protein coupled to the fluorescent dye ATTO 594, reactivates, boosts and stabilizes your GFP signal.
Application Notes For the immunofluorescence staining of GFP-fusion proteins in fixed cells

ATTO 594:
Excitation range (λabs= 601 nm)
Emission range (λfl= 627 nm)

Nano-Booster are specific GFP- or RFP-binding protein coupled to the superior fluorescent dyes from ATTO-TEC. With our Booster you reactivate, boost and stabilize the signals of your fluorescent proteins.

Assay Procedure
  • 1. Fixation: 4 % paraformaldehyde (PFA) or 1:10 formalin (37 % formaldehyde, 10-15 % MetOH) in PBS, 10 min., RT.
  • 2. Wash 3x with PBS containing 0.1 % Tween 20 (PBST). Critical: do not let coverslips ""dry"".
  • 3. Permeabilisation: PBS containing 0.5 % Triton X-100, 5 min., RT. Alternatively permeabilise by incubating in 100 % methanol for 5 min at -20 °C.
  • 4. Wash 2x with PBST.
  • 5. Blocking: 4 % BSA in PBST, 10 min, RT.
  • 6. GFP-Booster incubation: dilute GFP-Booster 1:200 in blocking buffer and incubate 1 h, RT. Note: For multiplexing protocols you can combine GFP-Booster with any other antibody.
  • 7. Wash 3x 5-10 min in PBST.
  • 8. If required counterstain with DNA fluorescent dyes, e.g. DAPI.
  • 9. Before mounting coverslips can be very briefly rinsed in water to prevent salt crystals to form.
  • 10. Mount in VectaShield (Vector Labs) or other mounting media with anti-fading agents and seal mounted coverslips with clear nail polish. Please note: Optimal dilutions/ concentrations should be determined by the end user
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.01 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze. Protect from light.
Storage 4 °C
Expiry Date 6 months
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