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10X PBS Buffer pH 7.2

ELISA, FACS, IF, WB 10 X
Rockland
Catalog No. ABIN925546
Supplier Product No.: mb-008
$97.00
Plus shipping costs $50.00
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Quick Overview for 10X PBS Buffer pH 7.2 (ABIN925546)

Application

ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
  • Supplier Product No.

    mb-008

    Supplier

    Rockland

    Purpose

    10X PBS Buffer pH 7.2

    Purification

    10X PBS buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
  • Application Notes

    Application Note: This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Ch

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    10 X

    Handling Advice

    Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature

    Storage

    RT,4 °C

    Storage Comment

    Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) Expiration date is six (6) months from date of receipt.

    Expiry Date

    6 months
  • Background

    Synonyms: Phosphate buffered saline, Phosphate buffered solution, PBS, 10X PBS

    Background: Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluores

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