RIPA Lysate Solution

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Cell Lysis (CLy), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
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Characteristics Extraction of cellular proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and/or interference with protein immunoreactivity and biological activity. RIPA (Radio-Immunoprecipitation Assay) Solution enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash solution for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this solution. In addition, RIPA Solution minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Product cited in: Lv, Sun, Ma, Ma, Zhang, Li, Li, Zhao: "Netrin-1 induces the migration of Schwann cells via p38 MAPK and PI3K-Akt signaling pathway mediated by the UNC5B receptor." in: Biochemical and biophysical research communications, Vol. 464, Issue 1, pp. 263-8, 2015 (PubMed).

Sun, Chen, Ma, Liang, Zheng, Guo, Wang, Wang, Li, Zhong: "Administration of SB203580, a p38 MAPK Inhibitor, Reduced the Expression of MMP9, and Relieved Neurologic Severity in the Experimental Autoimmune Neuritis (EAN) in Rats." in: Neurochemical research, Vol. 40, Issue 7, pp. 1410-20, 2015 (PubMed).

Guo, Jin, Fang, You, Wang, Hu, Han: "Beneficial effects of hydrogen-rich saline on early burn-wound progression in rats." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124897, 2015 (PubMed).

Xiao, Gao, Duan, Yuan, Ke: "Downregulation of miR-19a exhibits inhibitory effects on metastatic renal cell carcinoma by targeting PIK3CA and inactivating Notch signaling in vitro." in: Oncology reports, Vol. 34, Issue 2, pp. 739-46, 2015 (PubMed).

Wang, Xie, Wang, Li, Zhang, Liu, Hong, Du: "All-trans-retinoid acid (ATRA) suppresses chondrogenesis of rat primary hind limb bud mesenchymal cells by downregulating p63 and cartilage-specific molecules." in: Environmental toxicology and pharmacology, Vol. 38, Issue 2, pp. 460-8, 2014 (PubMed).

Wang, Dao, Bao, Dong, Wang, Han, Yue, Yu: "Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes." in: Journal of cellular and molecular medicine, Vol. 18, Issue 9, pp. 1807-15, 2014 (PubMed).

Tang, Yang, Zhang, Guo, Peng, Wang et al.: "Activation of extracellular signal-regulated kinase 1/2 and Sp1 may contribute to the expression of tissue inhibitor of metalloproteinases-1 induced by transforming growth factor-β1 in human ..." in: Cytotherapy, Vol. 16, Issue 2, pp. 225-33, 2014 (PubMed).

Dong, Wang, Tan, Li, Qian, Chen, Chen, Wang, Xu, Tao, Wang: "Decreased expression of microRNA-21 correlates with the imbalance of Th17 and Treg cells in patients with rheumatoid arthritis." in: Journal of cellular and molecular medicine, Vol. 18, Issue 11, pp. 2213-24, 2014 (PubMed).

Chen, Ma, Huang, Zhang, Zhong, Han, Hu, Li: "Efficiency of transcellular transport and efflux of flavonoids with different glycosidic units from flavonoids of Litsea coreana L. in a MDCK epithelial cell monolayer model." in: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Vol. 53, pp. 69-76, 2014 (PubMed).

Wang, Liao, Fan, Ye, Sun, Dong: "Apigenin inhibits the expression of IL-6, IL-8, and ICAM-1 in DEHP-stimulated human umbilical vein endothelial cells and in vivo." in: Inflammation, Vol. 35, Issue 4, pp. 1466-76, 2012 (PubMed).

Background publications Jia, Yang, Ren, Chen, Feng, Cao, Zhang, Li, Lei: "Tanshinone IIA reduces apoptosis induced by hydrogen peroxide in the human endothelium-derived EA.hy926 cells." in: Journal of ethnopharmacology, Vol. 143, Issue 1, pp. 100-8, 2012 (PubMed).

Huang, Lv, Luo, Liao, Zhu, Wang: "Identification, characterization and the interaction of Tollip and IRAK-1 in grass carp (Ctenopharyngodon idellus)." in: Fish & shellfish immunology, Vol. 33, Issue 3, pp. 459-67, 2012 (PubMed).

Zhang, Xu, Guo, Meng, Li: "Anti-diabetic effects of cinnamaldehyde and berberine and their impacts on retinol-binding protein 4 expression in rats with type 2 diabetes mellitus." in: Chinese medical journal, Vol. 121, Issue 21, pp. 2124-8, 2008 (PubMed).