Binding/Coating Buffer (10X)

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Characteristics BBC is a ten times concentrated liquid reagent for coating ELISA plates and other solid phase supports/matrices made of PS or like plastic materials primarily with IgG antibodies. BBC is applicable in both manual and robotic ELISA protocols. 1 Volume of BBC mixed with 9 Volumes of pure deionized water will give 10 Volumes of 50 mM Na-Carbonate/Bicarbonate Buffer pH 9.5 +/-0,03 containing 0,025% 5-Bromo-5-Nitro-1,3- dioxane which is an optimal universal Coating Buffer for antibodies and vast majority of protein antigens (except for very acidic proteins having pI smaller than 5,5) in ELISA and related tests that rely on physical adsorption of biomolecules onto hydrophobic and/or negatively charged plastic surfaces.
Sterility 0.2 μm filtered
Restrictions For Research Use only
Format Liquid
Concentration 10 X
Buffer Transparent liquid, 0.2 um filtered. Weak odor characteristic. Supplied as a liquid, pH 9.15, with 5-Bromo-5-nitro-1,3-dioxane as a preservative, 2500 ppm.
Preservative Bromonitrodioxane
Precaution of Use This product contains Bromonitrodioxane: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C