anti-Human Galanin Receptor 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Galanin Receptor 1 Antibody (supplied by: Log in to see )

Antigen
Galanin Receptor 1 (GALR1) Antibodies
  • GALNR
  • GALNR1
  • GALR1
  • GALR
  • Galnr1
  • galanin receptor 1
  • transcription regulator. LacI family, galactose related
  • LacI family transcriptional regulatory protein
  • galactose operon repressor
  • GALR1
  • galr1
  • galR
  • galR1
  • Galr1
  • LOC100037697
Epitope
N-Term
25
24
10
10
8
6
4
3
3
3
2
2
1
1
1
1
Reactivity
Human
95
22
16
2
2
2
2
1
1
1
1
Host
Rabbit
77
34
Clonality
Polyclonal
Conjugate
This Galanin Receptor 1 antibody is un-conjugated
6
6
6
5
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
63
60
23
19
13
10
5
4
3
2
1
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Catalog No. ABIN271021
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.390666 ABIN4313198 IHC (p) Rabbit 2nd Extracellular Domain Log in to see Polyclonal 0
12.390666 ABIN4313199 IHC (p) Rabbit C-Term Log in to see Polyclonal 0
12.390666 ABIN4273335 ICC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1048641 ICC IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1048642 IHC IHC (p) Rabbit Extracellular Domain Log in to see Polyclonal 0
1 ABIN1048640 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1804263 ELISA IHC IHC (p) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1734567 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1734566 IHC (p) Rabbit IgG 2nd Extracellular Domain Log in to see Polyclonal 0
1 ABIN1734568 ICC IHC (p) Rabbit IgG N-Term, Extracellular Domain Log in to see Polyclonal 0
1 ABIN1423360 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN1405489 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1385529 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4889837 IHC (p) ELISA Rabbit IgG AA 285-349 Log in to see Polyclonal 0
1 ABIN5556953 IHC (p) Rabbit Cytoplasmic Domain Log in to see Polyclonal 0
1 ABIN5556954 IHC (p) Rabbit N-Term Log in to see Polyclonal 0
1 ABIN5578610 IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN5578609 IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN5578608 ICC IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN2768007 IHC (p) IHC ELISA Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Galanin Receptor 1 (GALR1) Antibodies
Epitope N-Term
(25), (24), (10), (10), (8), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human
(95), (22), (16), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(77), (34)
Clonality
Polyclonal
Conjugate This Galanin Receptor 1 antibody is un-conjugated
(6), (6), (6), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(63), (60), (23), (19), (13), (10), (5), (4), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-Galanin Receptor 1 Antibody

Target Details Galanin Receptor 1 Application Details Handling References for anti-Galanin Receptor 1 antibody (ABIN271021) Images
Specificity Human GALR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SSTR5 (50 % ).
Purification Immunogen affinity purified
Immunogen Synthetic 18 amino acid peptide from N-terminal extracellular domain of human GALR1.
Plasmids, Primers & others

Target Details Galanin Receptor 1

Product Details anti-Galanin Receptor 1 Antibody Application Details Handling References for anti-Galanin Receptor 1 antibody (ABIN271021) Images back to top
Antigen
Alternative Name GALR1 (GALR1 Antibody Abstract)
Background Gene Symbol: GALR1
Gene ID 2587
UniProt P47211
Pathways cAMP Metabolic Process

Application Details

Product Details anti-Galanin Receptor 1 Antibody Target Details Galanin Receptor 1 Handling References for anti-Galanin Receptor 1 antibody (ABIN271021) Images back to top
Application Notes Immunohistochemistry-Paraffin 19 μg/mL, Immunocytochemistry 1:10 - 1:500ICC/IF usage is reported in scientific literature (PMID: 21821102).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for GALR1 Antibody (NLS4105)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only

Handling

Product Details anti-Galanin Receptor 1 Antibody Target Details Galanin Receptor 1 Application Details References for anti-Galanin Receptor 1 antibody (ABIN271021) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Galanin Receptor 1 antibody (ABIN271021)

Product Details anti-Galanin Receptor 1 Antibody Target Details Galanin Receptor 1 Application Details Handling Images back to top
Product cited in:

Barson, Chang, Poon, Morganstern, Leibowitz: "Galanin and the orexin 2 receptor as possible regulators of enkephalin in the paraventricular nucleus of the hypothalamus: relation to dietary fat." in: Neuroscience, Vol. 193, pp. 10-20, 2011

Images

Product Details anti-Galanin Receptor 1 Antibody Target Details Galanin Receptor 1 Application Details Handling References for anti-Galanin Receptor 1 antibody (ABIN271021) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Galanin Receptor 1 (GALR1) (N-Term) antibody (ABIN271021) Immunohistochemistry-Paraffin: GALR1 Antibody [NLS4105] - Formalin-fixed paraffin sec...