anti-Bat Mu Opioid Receptor 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Mu Opioid Receptor 1 Antibody (supplied by: Log in to see )

Antigen
Opioid Receptor, mu 1 (OPRM1) Antibodies
  • LMOR
  • M-OR-1
  • MOP
  • MOR
  • MOR1
  • OPRM
  • MOP-R
  • MOR-1
  • MOR-1O
  • Oprm
  • mor
  • muOR
  • MORA
  • Oprrm1
  • or2
  • ZFOR2
  • OPRM1
  • opioid receptor mu 1
  • opioid receptor, mu 1
  • outer membrane protein OprM
  • OPRM1
  • Oprm1
  • oprm1
  • oprM1
Epitope
C-Term
31
15
14
11
10
9
8
8
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Hamster, Human, Monkey, Pig (Porcine)
130
76
60
10
7
7
3
3
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
132
4
3
Clonality
Polyclonal
Conjugate
This Mu Opioid Receptor 1 antibody is un-conjugated
5
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
99
58
50
32
18
14
11
9
5
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1049171
$ 484.00
Plus shipping costs $45.00

General

Antigen Opioid Receptor, mu 1 (OPRM1) Antibodies
Epitope C-Term
(31), (15), (14), (11), (10), (9), (8), (8), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Hamster, Human, Monkey, Pig (Porcine)
(130), (76), (60), (10), (7), (7), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(132), (4), (3)
Clonality
Polyclonal
Conjugate This Mu Opioid Receptor 1 antibody is un-conjugated
(5), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(99), (58), (50), (32), (18), (14), (11), (9), (5), (2), (2), (1), (1)
Supplier Log in to see

Product Details anti-Mu Opioid Receptor 1 Antibody

Target Details Mu Opioid Receptor 1 Application Details Handling Images
Brand IHC-plus™
Specificity Human OPRM1 / Mu Opioid Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Hamster, Elephant, Panda, Pig (100%) Mouse, Horse, Rabbit, Opossum, Platypus (94%) Rat (89%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from C-terminus of human OPRM1 / Mu Opioid Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Hamster, Elephant, Panda, Pig (100%), Mouse, Horse, Rabbit, Opossum, Platypus (94%), Rat (89%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details Mu Opioid Receptor 1

Product Details anti-Mu Opioid Receptor 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name OPRM1 / mu Opioid Receptor (OPRM1 Antibody Abstract)
Background Name/Gene ID: OPRM1
Subfamily: Opioid
Family: GPCR

Synonyms: OPRM1, M-OR-1, LMOR, MOR, Mu-type opioid receptor, HMOP, Opioid mu receptor, MOP, MOR-1, MOR1, Mu opioid receptor, Opioid receptor, mu 1, KIAA0403, MOR-1C, Mu opiate receptor, Mu opioid receptor hMOR-1a, Opioid mu-type receptor, OPRM
Gene ID 4988
Pathways cAMP Metabolic Process, Synaptic Membrane

Application Details

Product Details anti-Mu Opioid Receptor 1 Antibody Target Details Mu Opioid Receptor 1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (4 - 22 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-Mu Opioid Receptor 1 Antibody Target Details Mu Opioid Receptor 1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Mu Opioid Receptor 1 Antibody Target Details Mu Opioid Receptor 1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Opioid Receptor, mu 1 (OPRM1) (C-Term) antibody (ABIN1049171) Anti-OPRM1 / Mu Opioid Receptor antibody ABIN1049171 IHC staining of human brain, ne...
Immunohistochemistry (IHC) image for anti-Opioid Receptor, mu 1 (OPRM1) (C-Term) antibody (ABIN1049171) Anti-OPRM1 / Mu Opioid Receptor antibody IHC of human Brain, Glioblastoma. Immunohist...